应用高效液相色谱法及薄层层析法对真菌融合菌株发酵液的鉴别
Differentiation of Fermentation Liquors of Fungus Crasis by HPLC and TLC
摘 要
为确定两株真菌形成的融合菌株的有效性,以融合菌株和双亲本发酵液为研究对象,进行提取、离心、浓缩等预处理后,采用高效液相色谱法及薄层层析法对真菌融合菌株发酵液成分进行分析.对比融合菌株与两株真菌亲本因降解能力的不同而使发酵液组分产生的差异,从而鉴别融合菌株形成的真伪.
薄层色谱法
融合菌株
鉴别
HPLC
TLC
Fungus crasis
Differentiation
Abstract
Samples of fermentation liquors of fungus carsis and their parents were taken for the study.The sample liquors were pretreated according to prescribed procedure including extraction,centrifuging,concentration and etc.An aliquot of the concentrated sample solution was used for HPLC and TLC determinations.It was found that due to the difference of degradation ability of fungus crasis and their parants in the fermented liquors,different degradation products were obtained in different sample liquors,on the base of which differentiation of fungus crasis was performed.
中图分类号 O652.63
所属栏目 工作简报
基金项目 辽宁科技厅科技攻关项目(2000050107),校青年基金(2004014)
收稿日期 2007/12/26
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备注刘玲(1973-),女,辽宁沈阳人,博士,副教授,从事食品生物技术和质量安全的教学和科研工作.
引用该论文: LIU Ling,LIU Chang-jiang,YE Bo. Differentiation of Fermentation Liquors of Fungus Crasis by HPLC and TLC[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(2): 138～140
刘玲,刘长江,叶博. 应用高效液相色谱法及薄层层析法对真菌融合菌株发酵液的鉴别[J]. 理化检验－化学分册, 2009, 45(2): 138～140
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