植物油或含动物油脂的样品用无水乙醇处理使其油脂完全溶解,分取部分试液供高效液相色谱法测定其胆固醇含量.用SB-C18分析柱(4.6 mm×150 mm)作为固定相,用甲醇与水(95+5)的混合液作流动相,其流速为1.0 mL·min-1,在208 nm波长处作紫外吸收检测.此方法的检出限(3S/N)为2.0 mg·L-1,方法的回收率在96.5%~99.0%之间.

Samples of vegetable or animal oil or fat were treated with absolute ethanol and aliquots of the solutions obtained were used for HPLC determination of cholesterol contents.Analytical column of SB-C18 (4.6 mm×150 mm) was used as the stationary phase and mixture of methanol and water (95+5) was used as the mobile phase at the rate of 1.0 mL·min-1.UV-detections were made at the wavelength of 208 nm.Detection limit (3S/N) of the method was found to be 2.0 mg·L-1.Recovery was tested and the results obtained were in the range of 96.5%-99.0%.