卡那霉素与苯胺蓝反应的电化学研究及其应用
Electrochemical Study on the Reaction of Kanamycin with Aniline Blue and Its Application
摘 要
在pH 3.0的B-R缓冲溶液中,苯胺蓝在静汞电极上于-0.355 V产生明显的还原峰.当加入卡那霉素之后,卡那霉素与苯胺蓝发生作用生成新的离子复合物,使苯胺蓝在-0.355 V处的还原峰电流下降,研究发现其降低值ΔIp与卡那霉素的质量浓度在5.0~65.0 mg·L-1范围内呈线性关系,线性回归方程为ΔIp=-1.028+0.567 C,相关系数为0.998,检出限(3S/N)为1.0 mg·L-1.用该法测定了市售硫酸卡那霉素的含量,所得结果与分光光度法的测定结果一致.
苯胺蓝
卡那霉素
Voltammetry
Aniline blue
Kanamycin
Abstract
In a B-R buffer solution of pH 3.0,a significant reduction peak of aniline blue with its potential at -0.355 V was observed at the static mercury electrode.Upon the addition of an appropriate amount of kanamycin to this reaction system,the peak current (Ip) at -0.355 V was decreased due to the reaction of aniline blue with kanamycin forming an ion-association complex.The magnitude of decrease in peak current (ΔIp) at -0.355 V was found to keep linear relationship with the concentration of kanamycin in the range from 5.0 to 65.0 mg·L-1,corresponding to the linear regression equation of ΔIp=-1.028+0.567 C with its correlation coefficient of 0.998.Detection limit (3S/N) found was 1.0 mg·L-1.The proposed method was used to determine content of kanamycin in samples of kanamycin injections,giving results in consistency with the results obtained by spectrophotometry.
中图分类号 O657.1
所属栏目 工作简报
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收稿日期 2007/11/22
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备注王学亮(1977-),男,山东东明人,讲师,硕士,主要从事电分析化学研究.
引用该论文: WANG Xue-liang,ZHAO Jie. Electrochemical Study on the Reaction of Kanamycin with Aniline Blue and Its Application[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(2): 207～209
王学亮,赵杰. 卡那霉素与苯胺蓝反应的电化学研究及其应用[J]. 理化检验－化学分册, 2009, 45(2): 207～209
被引情况:
【1】王学亮,王朝霞,蔡艳丽, "聚苯胺纳米管/壳聚糖修饰玻碳电极循环伏安法测定阿米卡星",理化检验－化学分册 47, 407-409(2011)
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参考文献
【1】湛海粼,胡小莉,刘绍璞,等.卡那霉素、新霉素对甲基蓝的褪色反应及其分析应用[J].西南师范大学学报:自然科学版,2004,29(1):106-108．
【2】江虹,胡小莉,刘绍璞,等.镧-滂胺天蓝光度法测定人血清及尿样中的几种抗生素[J].分析化学,2004,32(8):1043-1046．
【3】王明霞,刘忠芳,胡小莉,等.达旦黄与氨基糖苷类抗生素相互作用的分光光度研究及其分析应用[J].西南师范大学学报:自然科学版,2005,30(2):304-307．
【4】Rizk M,El-Shabrawy Y,Zakhari N A,et al.Fluorimetric determination of aminoglycoside antibiotics using lanthanide probe ion spectroscopy[J].Talanta,1995,42(12):1849-1856．
【5】Alykov N M.Extraction-fluorimetic determination of aminoglycosides antibiotics[J].Zh Anal Khim,1981,36(7):1287-1389．
【6】Clifton K F,Alan R L,Steven J L.Confirmatory and quantitative analysis of β-lactan antibiotics in bovine kidney tissue by dispersive solid phase extraction and liquuid chromatography tandem mass spectrometry[J].Anal Chem,2005,77:1473-1479．
【7】孙汝东,严金龙.卡那霉素注射液含量的方波伏安法测定[J].分析测试学报,2001,20(5):83-85．
【8】王学亮.以鸡冠花红为电化学探针线性扫描极谱法测定阿米卡星[J].分析试验室,2006,25(6):43-45．
【9】Wang X L,Yu Z Y,Jiao K.Voltammetric studies on the interaction of amikacin with methyl blue and its analytical application[J].Chin Chem Lett,2007,18:94-96．
【10】江虹.水溶苯胺蓝褪色光度法测定庆大霉素及妥布霉素的研究[J].分析科学学报,2005,21(3):301-303．
【2】江虹,胡小莉,刘绍璞,等.镧-滂胺天蓝光度法测定人血清及尿样中的几种抗生素[J].分析化学,2004,32(8):1043-1046．
【3】王明霞,刘忠芳,胡小莉,等.达旦黄与氨基糖苷类抗生素相互作用的分光光度研究及其分析应用[J].西南师范大学学报:自然科学版,2005,30(2):304-307．
【4】Rizk M,El-Shabrawy Y,Zakhari N A,et al.Fluorimetric determination of aminoglycoside antibiotics using lanthanide probe ion spectroscopy[J].Talanta,1995,42(12):1849-1856．
【5】Alykov N M.Extraction-fluorimetic determination of aminoglycosides antibiotics[J].Zh Anal Khim,1981,36(7):1287-1389．
【6】Clifton K F,Alan R L,Steven J L.Confirmatory and quantitative analysis of β-lactan antibiotics in bovine kidney tissue by dispersive solid phase extraction and liquuid chromatography tandem mass spectrometry[J].Anal Chem,2005,77:1473-1479．
【7】孙汝东,严金龙.卡那霉素注射液含量的方波伏安法测定[J].分析测试学报,2001,20(5):83-85．
【8】王学亮.以鸡冠花红为电化学探针线性扫描极谱法测定阿米卡星[J].分析试验室,2006,25(6):43-45．
【9】Wang X L,Yu Z Y,Jiao K.Voltammetric studies on the interaction of amikacin with methyl blue and its analytical application[J].Chin Chem Lett,2007,18:94-96．
【10】江虹.水溶苯胺蓝褪色光度法测定庆大霉素及妥布霉素的研究[J].分析科学学报,2005,21(3):301-303．
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