一种新的分子内电荷转移荧光探针测定牛血清白蛋白
Determination of Bovine Serum Albumin by a New Intramolecular Charge Transfer Fluorescence Probe
摘 要
基于一种新的分子内电荷转移荧光探针1-酮-2-(对二甲氨基苯亚甲基)-四氢萘(简称KDTN)与牛血清白蛋白的结合反应,提出了测定牛血清白蛋白的荧光分光光度法.在激发波长(λex)为430 nm,最大发射波长(λem)为530 nm及选定的试验条件下,测定牛血清白蛋白的线性范围为6.8~204.0 mg·L-1,线性回归方程ΔF=0.583 6 ρ-1.791 7,相关系数为0.999 1,方法的检出限(3S/N)为0.4 mg·L-1,样品的加标回收率为99.9%~102.0%.
荧光探针
1-酮-2-(对二甲氨基苯亚甲基)-四氢萘
牛血清白蛋白
Intramolecular charge transfer
Fluorescence probe
1-Keto-2-(p-dimethylaminobenzal)-tetrahydronaphthalene
Bovine serum albumin
Abstract
Based on the combination reaction between bovine serum albumin (BSA) and the fluorescence probe,1-keto-2-(p-dimethylaminobenzal)-tetrahydronaphthalene (KDTN),through intramolecular charge transfer,a new method of fluorospectrophotometric determination of BSA was proposed.Under the optimum condition,fluorescence detection was made at the wavelengths of (λex) 438 nm and (λem) 530 nm.Linear relationship between values of fluorescence intensity (F) and concentration of BSA was kept in the range of 6.8-204.0 mg·L-1,corresponding to the linear regression equation ΔF=0.583 6 ρ-1.791 7 (r=0.999 1).Detection limit (3S/N) of the method was found to be 0.4 mg·L-1.Test for recovery was made by standard addition method,giving values of recovery in the range of 99.9%-102.0%.
中图分类号 O657.31
所属栏目 试验与研究
基金项目 国家自然科学基金(20575037),山西省自然科学基金(20051012)资助项目
收稿日期 2008/6/30
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备注刘毓芳(1963-),女,山西文水人,副教授,硕士,主要研究方向为分子光谱分析.
引用该论文: LIU Yu-fang,LI Jan-qing,DONG Chuan. Determination of Bovine Serum Albumin by a New Intramolecular Charge Transfer Fluorescence Probe[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(3): 261～263
刘毓芳,李建晴,董川. 一种新的分子内电荷转移荧光探针测定牛血清白蛋白[J]. 理化检验－化学分册, 2009, 45(3): 261～263
被引情况:
【1】刘毓芳,李建晴,董川, "以1-酮-2-(对二甲氨基苯亚甲基)-四氢萘与β-环糊精的包合物为荧光探针测定牛血清白蛋白",理化检验－化学分册 50, 444-446(2014)
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参考文献
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【4】栾吉梅,张晓东.有机染料共振光散射法测定蛋白质的研究进展[J].理化检验-化学分册,2007,43(2):159-162．
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【6】Wang S L,Ho T I.Substituent effects on intramolecular charge-transfer behaviour of styrylheterocycles[J].J Photochem Photobiol A: Chem,2000,135(2):119-126．
【7】Lee J,Ross R T.Absorption and fluorescence of tyrosine hydrogen-bonded to amide-like ligands[J].J Phys Chem B,1998,102(23):4612-4618．
【8】Lee I H,Pinto D,Arriaga E A,et al.Picomolar analysis of proteins using electrophoretically mediated microanalysis and capillary electrophoresis with laser-induced fluorescence detection[J].Anal Chem,1998,70(21):4546-4548．
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