高效液相色谱法测定水果和蔬菜中多菌灵残留量
HPLC Determination of Residual Carbendazim in Fruits and Vegetables
摘 要
应用高效液相色谱法测定了水果和蔬菜中多菌灵的残留量.样品用快速溶剂萃取仪提取后,经浓缩、净化处理,所得残渣用流动相混合液溶解,通过C18色谱柱分离.以pH 6.8的磷酸盐缓冲溶液与乙腈以80比20(体积比)混合后作为流动相,流速为1.0 mL·min-1,在286 nm波长处,用二极管阵列检测器检测.多菌灵的质量浓度与其峰面积在0.05~10.0 mg·L-1范围内呈线性关系,方法的测定下限(10S/N)为0.02 μg·g-1.以大白菜、番茄、苹果、梨样品为基体,加入标准溶液进行回收试验,测得回收率在81.4%~95.8%之间.
水果和蔬菜
多菌灵残留量
HPLC
Fruits and vegetables
Carbendazim residues
Abstract
High performance liquid chromography was applied to the determination of residual carbendazim in fruits and regetables.The sarple was extracted by rapid solvent extraction,and the extract was concentrated and purified.The residue obtained was dissolved in the mobile-phase solution and separated on the C18 column.A mixture of PBS (pH 6.8) and acetonitrile mixed in the ratio of 80 to 20 (by volume) was used as mobile-phase at a flow-rate of 1.0 mL·min-1.UV-detection at 286 nm with photo diode array was used in the determination.Linear relationship between values of peak area and mass concentration of carbendazim was kept in the range of 0.05-10.0 mg·L-1.Lower limit of determination (10S/N) of the method was found to be 0.02 μg·g-1.On the base of samples of cabbage,tomao,apple and peach,tests for recovery were made by standard addition method,values of recovery found were in the range of 81.4%-95.8%.
中图分类号 O657.37
所属栏目 工作简报
基金项目
收稿日期 2008/8/15
修改稿日期
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备注卢业举(1967-),男,安徽庐江人,高级工程师,研究方向为食品和农产品的品质分析和研究.
引用该论文: LU Ye-ju,CHENG Jing,NIE Lei. HPLC Determination of Residual Carbendazim in Fruits and Vegetables[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(3): 296～298
卢业举,程静,聂磊. 高效液相色谱法测定水果和蔬菜中多菌灵残留量[J]. 理化检验－化学分册, 2009, 45(3): 296～298
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参考文献
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【3】徐宝才,岳永德,胡颍蕙,等.多菌灵的光化学降解研究[J].分析测试学报,2000,20(5):616-620．
【4】葛兴,谭永强,周小虹,等.一种测定番茄中多菌灵残留动态的有效方法[J].北京农学院学报,1997,12(1):78-82．
【5】李桂凤,郝征红.花生中多菌灵残留量的高效液相色谱分析方法的研究[J].分析测试学报,1998,17(3):69-70．
【6】孔祥虹,何学文,张妮华,等.高效液相色谱法测定浓缩苹果汁中的多菌灵残留量[J].化学分析计量,2003,12(1):13-15．
【7】郝金芝.高效液相色谱法测定水果中多菌灵[J].中国卫生检验杂志,2004,14(5):586-587．
【8】李桂凤,郝征红.花生中多菌灵残留量的高效液相色谱分析方法的研究[J].分析测试学报,1998,17(3):69-70．
【9】高蓉,赵人琤,杨俊,等.高效液相色谱法测定多菌灵在油菜籽中的残留量[J].南京医科大学学报,2004,24(4):425-426．
【10】郭英娜,姜茁松,张敏,等.固相萃取富集-高效液相色谱法测定环境水中多菌灵和噻菌灵[J].分析化学,2005,33(3):395-397．
【11】季凤英,李晓东,陈瑞耘,等.桑叶中多菌灵的紫外光谱法分析[J].中山大学学报:自然科学版,1995,34(1):118-121．
【12】Margareta Sandahl,Lennart Mathiasson,Jan Ake Jenison.Determination of thiophanate-methyl and itsmetabolites at trace level in spiked natural water using the supported liquid membrane extraction and the microporous membrane liquid-liquid extraction techniques combined on-line with high-performance liquid chromatography[J].Journal of Chromatography A,2000,893:123-131．
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