在pH 10的磷酸盐缓冲介质中,十二烷基苯磺酸钠(SDBS)对小檗碱的荧光有明显的增敏作用,且其荧光的增强程度与小檗碱浓度在2.0×10-9~1.0×10-3mol·L-1之间呈线性关系.此反应对小檗碱的检出限(3S/N)为1.29×10-9mol·L-1.利用此反应作为测定小檗碱的方法并应用于小檗碱片剂的分析,测得结果与药典法测定结果相符,对药片的标示量而言,测定所得回收率的平均值为99.8%,测定结果的相对标准偏差(n=5)的平均值为0.17%.

In a phosphate buffer medium of pH 10,sensitization effect of sodium dodecyl benzene sulfonate (SDBS) on the fluorescence of berberine was observed obviously,and the magnitude of increase in fluorescence intensity was found to keep linear relationship with the concentration of berberine in the range of 2.0×10-9-1.0×10-3mol·L-1.Detection limit (3S/N) of the reaction was found to be 1.29×10-9mol·L-1.The reaction was used as a method to determine berberine and was applied to the analysis of berberine tablets.Results obtained were in conformity with those obtained by the pharmacopoeia method with average values of recovery and RSD′s (n=5) 99.8% and 0.17% respectively.