大豆黄素的电化学行为及其循环伏安测定
Electrochemical Behavior of Daidzein and Its Voltammetric Determination
摘 要
在pH 7.5的三羟甲基氨基甲烷-盐酸(Tris-盐酸)缓冲溶液的底液中,采用循环伏安法测定大豆黄素,得到一良好的氧化峰,其峰电位在0.53 V处;峰电流与大豆黄素的浓度在1.2×10-7~9.6×10-6mol·L-1范围内呈线性关系.该方法的检出限(3S/N)为8.0×10-8mol·L-1.用此法测定红车轴草中大豆黄素含量,加标平均回收率为98.0%;对1.6×10-6mol·L-1大豆黄素平行测定8次,进行精密度试验,相对标准偏差为0.81%.根据大豆黄素在玻碳电极上的电化学行为,表明大豆黄素的电极过程具有吸附性和不可逆性.
电化学行为
大豆黄素
Cyclic voltammetry
Electrochemical behavior
Daidzein
Abstract
In a Tris-HCl buffer base solution of pH 7.5,a well-defined oxidation peak with its peak potential at 0.53 V (vs.AgCl/Ag) was observed in the cyclic voltammogram of daidzein.Linear relationship was obtained between values of peak current and concentration of daidzein in the range of 1.2×10-7-9.6×10-6mol·L-1.Detection limit (3S/N) of 8.0×10-8mol·L-1 was found for this method.Daidzein content in a sample of trifolium was determined by this method.Using this sample as matrix,recovery test was made by standard addition method,an average recovery of 98.0% was found.Test for precision was performed with 1.6×10-6mol·L-1 of daidzein,the value of RSD (n=8) found was 0.81%.It was added that the reaction of daidzein at the glassy carbon electrode was an adsorptive and irreversible process.
中图分类号 O657.1
所属栏目 工作简报
基金项目
收稿日期 2007/12/19
修改稿日期
网络出版日期
作者单位点击查看
备注黄宝美(1979-),女,山东临沂人,讲师,主要研究方向为电化学分析.
引用该论文: HUANG Bao-mei,YAO Cheng-wei,BIAN Qing-quan,WANG Xiu-feng,WANG Zhi-guo. Electrochemical Behavior of Daidzein and Its Voltammetric Determination[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(3): 319～320
黄宝美,姚程炜,边清泉,王秀峰,王志国. 大豆黄素的电化学行为及其循环伏安测定[J]. 理化检验－化学分册, 2009, 45(3): 319～320
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】吴周和,周丽明,张勇,等.反相高效液相色谱法测定豆制品中两种异黄酮甙元的含量[J].分析科学学报,2006,22(3):303-305．
【2】秦恩华.反相高效液相色谱法测定红车轴草提取物中异黄酮含量的研究[J].安徽农业科学,2006,34(12):2646-2647．
【3】Liselotte Krenn,Iris Unterrieder,Renate Ruprechter.Quantification of isoflavones in red clover by high-performance liquid chromatography[J].Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences,2002,777(1/2):123-129．
【4】Kuo Hanwen,Ding Wanghsien.Trace determination of bisphenol a and phytoestrogens in infant formula powders by gas chromatography-mass spectrometry[J].Journal of Chromatography A,2004,1027(1/2):67-53．
【5】Peng Y Y,Ye J N.Determination of isoflavones in red clover by capillary electrophoresis with electrochemical detection[J].Fitoterapia,2006,77(3):171-178．
【6】Li Cunhong,Chen Anjia,Chen Xiaofeng,et al.Separation and simultaneous determination of rutin,puerarin,daidzein,esculin and esculetin in medicinal preparations by non-aqueous capillary[J].Journal of Pharmaceutical & Biomedical Analysis,2005,39(1/2):125-131．
【7】Peng Youyuan,Chu Qingcui,Liu Fanghua,et al.Determination of isoflavones in soy products by capillary electrophoresis with electrochemical detection[J].Food Chemistry,2004,87(1):135-140．
【8】Cao Yuhua,Lou Changgang,Zhang Xin,et al.Determination of puerarin and daidzein in puerariae radix and its medicinal preparations by micellar electrokinetic capillary chromatography with electrochemical detection[J].Anal Chim Acta,2002,452(1):123-129．
【9】Mallis Larry M,Sarkahian Ani B,Harris Heather A,et al.Determination of rat oral bioavailability of soy-derived phytoestrogens using an automated on-column extraction procedure and electrospray tandem mass spectrometry[J].Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences,2003,796(1):71-87．
【2】秦恩华.反相高效液相色谱法测定红车轴草提取物中异黄酮含量的研究[J].安徽农业科学,2006,34(12):2646-2647．
【3】Liselotte Krenn,Iris Unterrieder,Renate Ruprechter.Quantification of isoflavones in red clover by high-performance liquid chromatography[J].Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences,2002,777(1/2):123-129．
【4】Kuo Hanwen,Ding Wanghsien.Trace determination of bisphenol a and phytoestrogens in infant formula powders by gas chromatography-mass spectrometry[J].Journal of Chromatography A,2004,1027(1/2):67-53．
【5】Peng Y Y,Ye J N.Determination of isoflavones in red clover by capillary electrophoresis with electrochemical detection[J].Fitoterapia,2006,77(3):171-178．
【6】Li Cunhong,Chen Anjia,Chen Xiaofeng,et al.Separation and simultaneous determination of rutin,puerarin,daidzein,esculin and esculetin in medicinal preparations by non-aqueous capillary[J].Journal of Pharmaceutical & Biomedical Analysis,2005,39(1/2):125-131．
【7】Peng Youyuan,Chu Qingcui,Liu Fanghua,et al.Determination of isoflavones in soy products by capillary electrophoresis with electrochemical detection[J].Food Chemistry,2004,87(1):135-140．
【8】Cao Yuhua,Lou Changgang,Zhang Xin,et al.Determination of puerarin and daidzein in puerariae radix and its medicinal preparations by micellar electrokinetic capillary chromatography with electrochemical detection[J].Anal Chim Acta,2002,452(1):123-129．
【9】Mallis Larry M,Sarkahian Ani B,Harris Heather A,et al.Determination of rat oral bioavailability of soy-derived phytoestrogens using an automated on-column extraction procedure and electrospray tandem mass spectrometry[J].Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences,2003,796(1):71-87．
相关信息
