采用基质固相分散法提取净化烟草中的阴离子,并用离子色谱法进行测定.将烟草样品与石墨化碳黑球(质量比为1比4)充分研磨后装柱,用热水洗脱,洗脱液中的阴离子用离子色谱测定.以YSA8型8164A-8阴离子色谱柱为固定相,用碳酸钠和碳酸氢钠混合液为流动相.6种阴离子F-、Cl-、NO-2、HPO2-4、NO-3和SO2-4在12 min内可完全分离;方法加标回收率在96.4%~103.6%之间,相对标准偏差(n=5)均小于2.1%,检出限(3S/N)均小于0.025 mg·L-1.

Six anions (i.e.,F-,Cl-,NO-2,HPO2-4,NO-3 and SO2-4) in tobacco sample were separated by the method of matrix solid phase dispersion and determined by ion chromatography (IC).0.1 g of the sample was mixed with 0.4 g of graphitized carbon black granules and ground in a mortar to make it a homogeneous dispersion.The dispersion was then filled into a solid phase extraction column.The SPE column was eluted with hot water to dissolve the anions from the sample in the dispersion.An aliquot of the eluate was used for IC determination.YSA8-8164A-8 Anion chromatographic column was used as stationary phase,and a mixed solution of Na2CO3 and NaHCO3 was used as mobile phase.Conductivity detection was adopted.The above mentioned anions were separately within 12 min.Values of D.L.(3S/N) were below 0.025 mg·L-1.Tests for recovery and precision were made,giving values of recovery ranged from 96.4%-103.6%,and of RSD′s (n=5) less than 2.1%.