壳聚糖-纳米金-葡萄糖氧化酶生物传感器的制备及其应用于葡萄糖的测定
Preparation of the Biosensor CHIT/nano Au/GOx and Its Application to Determination of Glucose
摘 要
采用了一种简便快捷的电沉积方法制备了壳聚糖-纳米金复合膜并应用于葡萄糖生物传感器的构建.氯金酸和壳聚糖的混合液在玻碳电极表面电化学还原为金纳米粒子,再将葡萄糖氧化酶通过戊二醛交联的方式固定在纳米金复合膜修饰的玻碳电极表面,制成一种新型的葡萄糖氧化酶生物传感器.该传感器对葡萄糖的响应十分快速,在5 s内即达到平衡.测定葡萄糖的线性范围为20 μmol·L-1~5 mmol·L-1,检出限(3S/N)为12 μmol·L-1.
壳聚糖
纳米金
葡萄糖
Glucose oxidase biosensor
Chitosan
Nanogold
Glucose
Abstract
Chitosan-nanogold modified glassy carbon electrode (CHIT/nano Au/GCE) was prepared by electrodeposition of nanogold particles from a mixed solution containing chitosan and HAuCl4 onto the surface of a pretreated GCE.And the biosensor (CHIT/nano Au/GOx) was prepared by immobilizaing glucose oxidase on the surface of CHIT/nano Au/GCE through cross-linking by pentanedial.This new biosensor behaved to give quick response to glucose (<5 s).Linear relationship between values of response current and concentration of glucose was kept in the range of 20 μmol·L-1-5 mmol·L-1,with a detection limit (3S/N) of 12 μmol·L-1.
中图分类号 O657.1
所属栏目 试验与研究
基金项目
收稿日期 2008/7/29
修改稿日期
网络出版日期
作者单位点击查看
备注戴松林(1956-),男,湖南省湘潭县人,高级工程师,主要从事环境监测、环境研究等工作.
引用该论文: DAI Song-lin,NI Na,WANG Ying,MAI Hui-min. Preparation of the Biosensor CHIT/nano Au/GOx and Its Application to Determination of Glucose[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(4): 384～386
戴松林,妮娜,王莹,麦慧敏. 壳聚糖-纳米金-葡萄糖氧化酶生物传感器的制备及其应用于葡萄糖的测定[J]. 理化检验－化学分册, 2009, 45(4): 384～386
被引情况:
【1】杨冬芝,孙世安,汤道权,徐淑坤,廉淑芹,王卿, "纳米金光度法测定药物中盐酸小檗碱",理化检验－化学分册 46, 598-600(2010)
【2】汪涛,王朝霞,王学亮,郁章玉, "基于血红蛋白/金纳米粒子/聚二烯丙基二甲基氯化铵-多壁碳纳米管生物传感器测定过氧化氢",理化检验－化学分册 49, 577-579(2013)
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】KARYAKIN A A,KARYAKINA E E,GORTON L.Prussian-blue-based amperometric biosensors in flow-injection analysis[J].Talanta,1996,43:1597-1606．
【2】GARJONYTE R,MALINAUSKAS A.Glucose biosensor based on glucose oxidase immobilized in electropolymerized polypyrrole and poly (o-phenylenediamine) films on a prussian blue-modified electrode[J].Sens Actuators B,2000,63:122-128．
【3】PAN D W,CHEN J H,NIE L H,et al.An amperometric glucose biosensor based on poly (o-aminophenol) and prussian blue films at platinum electrode[J].Anal Biochem,2004,324:115-122．
【4】YANG H,CHUNG T D,KIM Y T,et al.Glucose sensor using a microfabricated electrode and electropolymerized bilayer films[J].Biosens Bioelectron,2002,17:251-259．
【5】GOLE A,DASH C,RAMAKRISHNAN V,et al.Pepsin-gold colloid conjugates:preparation,characterization,and enzymatic activity[J].Langmuir,2001,17:1674-1682．
【6】XIAO Y,JU H X,CHEN H Y.Hydrogen peroxide sensor based on horseradish peroxidase-labeled Au colloids immobilized on gold electrode surface by cysteamine monolayer[J].Anal Chim Acta,1999,391:73-82．
【7】JIA J,WANG B,WU A,et al.A method to construct a third-generation horseradish peroxidase biosensor:self-assembling gold nanoparticles to three-dimensional sol-gel network[J].Anal Chem,2002,74:2217-2223．
【8】ZHOU Y,YUAN R,CHAI Y,et al.A reagentless amperometric immunosensor based on gold nanoparticles/thionine/Nafion-membrane-modified gold electrode for determination of α-1-fetoprotein[J].Electrochem Commun,2005,7:355-360．
【9】NIAZOV T,PAVLOV V,XIAO Y,et al.DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity[J].Nano Lett,2004,4:1683-1687．
【10】MIAO Y Q,TAN S W.Amperometric hydrogen peroxide biosensor based on immobilization of peroxidase in chitosan matrix crosslinked with glutaraldehyde[J].Analyst,2000,125:1591-1594．
【11】OKUMA H,WATANABE E.Flow system for fish freshness determination based on double multi-enzyme reactor electrodes[J].Biosens Bioelectro,2002,17(5):367-372．
【12】HUANG H,YANG X.Chitosan mediated assembly of gold nanoparticles multiplayer[J].Colloids Surf A,2003,226:77-86．
【2】GARJONYTE R,MALINAUSKAS A.Glucose biosensor based on glucose oxidase immobilized in electropolymerized polypyrrole and poly (o-phenylenediamine) films on a prussian blue-modified electrode[J].Sens Actuators B,2000,63:122-128．
【3】PAN D W,CHEN J H,NIE L H,et al.An amperometric glucose biosensor based on poly (o-aminophenol) and prussian blue films at platinum electrode[J].Anal Biochem,2004,324:115-122．
【4】YANG H,CHUNG T D,KIM Y T,et al.Glucose sensor using a microfabricated electrode and electropolymerized bilayer films[J].Biosens Bioelectron,2002,17:251-259．
【5】GOLE A,DASH C,RAMAKRISHNAN V,et al.Pepsin-gold colloid conjugates:preparation,characterization,and enzymatic activity[J].Langmuir,2001,17:1674-1682．
【6】XIAO Y,JU H X,CHEN H Y.Hydrogen peroxide sensor based on horseradish peroxidase-labeled Au colloids immobilized on gold electrode surface by cysteamine monolayer[J].Anal Chim Acta,1999,391:73-82．
【7】JIA J,WANG B,WU A,et al.A method to construct a third-generation horseradish peroxidase biosensor:self-assembling gold nanoparticles to three-dimensional sol-gel network[J].Anal Chem,2002,74:2217-2223．
【8】ZHOU Y,YUAN R,CHAI Y,et al.A reagentless amperometric immunosensor based on gold nanoparticles/thionine/Nafion-membrane-modified gold electrode for determination of α-1-fetoprotein[J].Electrochem Commun,2005,7:355-360．
【9】NIAZOV T,PAVLOV V,XIAO Y,et al.DNAzyme-functionalized Au nanoparticles for the amplified detection of DNA or telomerase activity[J].Nano Lett,2004,4:1683-1687．
【10】MIAO Y Q,TAN S W.Amperometric hydrogen peroxide biosensor based on immobilization of peroxidase in chitosan matrix crosslinked with glutaraldehyde[J].Analyst,2000,125:1591-1594．
【11】OKUMA H,WATANABE E.Flow system for fish freshness determination based on double multi-enzyme reactor electrodes[J].Biosens Bioelectro,2002,17(5):367-372．
【12】HUANG H,YANG X.Chitosan mediated assembly of gold nanoparticles multiplayer[J].Colloids Surf A,2003,226:77-86．
相关信息
