酶催化分光光度法测定过氧化氢
Enzyme Catalytic Spectrophotometric Determination of Hydrogen Peroxide
摘 要
过氧化氢能够氧化茜素红使之褪色,而模拟酶-血红蛋白对其具有催化作用.建立了一种以茜素红为指示剂的过氧化氢-茜素红-血红蛋白酶催化反应体系测定痕量过氧化氢的方法.确定了反应的最佳条件,体系的酸度为pH 9.8的氨水-氯化铵缓冲溶液,最大吸收波长为525 nm.线性范围为3.0×10-7~8.0×10-5mol·L-1,检出限(3s/k)为5.2×10-8mol·L-1,表观摩尔吸光率为1.1×104L· mol-1·cm-1.该方法可用于雨水及消毒水中过氧化氢含量的测定.并以此样品为基体,测定了方法的平均回收率和相对标准偏差(n=5)依次为100.3%及3.26%.
酶催化
过氧化氢
血红蛋白
茜素红
Spectrophotometry
Enzyme catalysis
Hydrogen peroxide
Haemoglobin
Alizarin red
Abstract
It was found that the oxidation of alizarin red by hydrogen peroxide was catalyzed enzymatically by haemoglobin in an ammoniacal buffer solution of pH 9.8,leading to decoloration of alizarin red.Absorption maximum of the reaction system was found at the wavelength of 525 nm.Linear relationship was kept in the range of 3.0×10-7-8.0×10-5mol·L-1 between values of decrease in absorbance (ΔA) and concentration of hydrogen peroxide.Apparent molar absorptivity (ε525) and detection limit (3s/k) were found to be 1.1×104L· mol-1·cm-1 and 5.2×10-8mol·L-1 respectively.Base on these facts,the method mentioned above was proposed for determination of trace amounts of hydrogen peroxide.Tests for average recovery and precision of the method were peroformed on the base of samples of rainwater and disinfactant liquors,average values of recovery and RSD′s (n=5) obtained were 100.3% and 3.26% respectively.
中图分类号 O657.32
所属栏目 工作简报
基金项目 河南省教育厅自然科学基金资助项目(No.2007150048和No.2008A150029)
收稿日期 2008/2/25
修改稿日期
网络出版日期
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备注陈亚红(1975-),女,河南鲁山人,博士,副教授,主要从事分析化学教学及分子光谱的研究.
引用该论文: CHEN Ya-hong,LIU Hong-mei,TIAN Feng-shou,SONG Mao-ping,CHEN Peng-li. Enzyme Catalytic Spectrophotometric Determination of Hydrogen Peroxide[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(4): 401～403
陈亚红,刘红梅,田丰收,宋毛平,陈鹏丽. 酶催化分光光度法测定过氧化氢[J]. 理化检验－化学分册, 2009, 45(4): 401～403
被引情况:
【1】黄佳,常青,江国栋,邱阳,唐和清, "分光光度法间接快速测定过氧化氢——基于纳米α-FeOOH/GO的类似过氧化物酶的催化作用",理化检验－化学分册 50, 417-420(2014)
【2】倪鹏,江涛,施锦辉,张晓冬,倪天宸,李阳,吴忧凡,王金娟, "石墨烯/酶纳米复合多层膜修饰电极测定食品中的过氧化氢含量",理化检验－化学分册 52, 648-651(2016)
【3】唐宁莉,蒙华毅,李欣,蒋小艳, "牛血红蛋白催化分光光度法测定过氧化氢",理化检验－化学分册 50, 191-194(2014)
【4】江涛,倪鹏,施锦辉,张晓冬,倪天宸,王金娟,李阳,吴忧凡, "金纳米团簇荧光材料的制备及其应用于测定食品中的过氧化氢",理化检验－化学分册 52, 506-509(2016)
【5】唐宁莉,夏海鸣,蒙兴龙,凌悦菲,唐建振, "催化荧光光度法测定痕量过氧化氢",理化检验－化学分册 48, 682-684(2012)
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参考文献
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【2】刘长增,徐文军,魏乃静.1,5-二(2-羟基-5-磺酸基)-3-氰基甲催化褪色光度法测定微量H2O2[J].冶金分析,2006,27(2):70-72．
【3】庄会荣.靛红褪色光度法测定H2O2[J].理化检验-化学分册,2000,36(1):37,41．
【4】HUANG Xue-mei,ZHU Min,MAO Lu-yuan.Catalytic determination of hydrogen peroxide by using the molybdenum-porphyrin complex as mimetic enzyme of peroxidase[J].Anal Sci,1997,13(1):145-147．
【5】MIFUNE M,MUKUNO T,TANI M.Flow analysis of hydrogen peroxide with a dye-formation reaction catalyzed by an ion-exchange resin modified with Mn3+-tetrakis(sulfophenyl) porphine[J].Anal Sci,1998,14(3):519-522．
【6】CHASSEMPOUR A,DANESHFAR R,MOSSEVI R.Rapid and sensitive determination of cyclosporins by ryrolysis mass spectrometry[J].Anal Sci,1999,32(3):457-461．
【7】TANG Bo,DU Ming,SHEN Han-xi.The study and application of biomimic peroxidase ferric 2-hydroxy-1-naphthaldehyde thiosemic-carbazone (Fe3+-HNT)[J].Talanta,1998,47(2):361-366．
【8】ZHANG Ke,MAO Lu-yuan,CAI Ru-xiu.Stopped-flow spectrophotometric determination of hydrogen peroxide with hemoglobin as catalyst[J].Talanta,2000,51(1):179-186．
【9】王全林,刘志洪,蔡汝秀,等.血红蛋白作为催化剂高灵敏测定过氧化氢[J].分析化学,2002,30(8):928-931．
【10】BARHAN D,TRINDER P.An improved colour reagent for the determination of blood glucose by the oxidase system[J].Analyst,1972,97(2):142-145．
【11】TAMAOKU K,MUARO Y,OHKURA Y.New water-soluable hydrogen donors for the enzymatic spectrophotometric determination of hydrogen peroxide[J].Anal Chim Acta,1982,136(1):121-127．
【12】CLAPP P A,ERANS D F.Spectrophotometric determination of hydrogen peroxide with leuco Patent Blue Violet[J].Anal Chim Acta,1991,243(2):217-220．
【13】付崇岗,苏昌华.流动注射掺杂普鲁士蓝碳糊电极测定过氧化氢[J].分析科学学报,2005,21(3):256-258．
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