Photometric Determination of Gatifloxacin by the Decoloration Reaction with Congo Red
摘 要
试验表明:在微酸性或近中性的缓冲介质中加替沙星(GTXC)与刚果红(CGR)反应生成络合物导致后者的颜色减退.在pH 2.4~4.2的微酸性条件下反应时,其最大褪色波长位于616 nm处,在此波长测定时其线性范围在(0.05~2.98)×10-5mol·L-1之间,表观摩尔吸光率为1.84×104L·mol-1·cm-1;而在近中性的缓冲介质中(pH 4.5~7.5)反应时,最大褪色波长为508 nm,在此波长测定时其线性范围在(0.05~2.50)×10-5mol·L-1之间,表观摩尔吸光率为1.83×104L·mol-1·cm-1.根据结果提出了在不同酸度条件下GTXC的光度测定法,取3种市购GTXC注射液作为样品,按所提出的方法测定其中GTXC的含量,所得测定值与标示值相符.以此样品为基体加入GTXC标准溶液作方法的回收试验,测得回收率在98.5%~100.4%之间.
Abstract
It was found that a complex was formed by the reaction of gatifloxacin (GTXC) with congo red (CGR),leading to decoloration of congo red in a slightly acidic buffer medium or in a nearly neutral buffer medium.Linear relationships were obtained between the values of decrease in absorbance and concentration of GTXC in the range of (0.05-2.98)×10-5mol·L-1,when reacted in pH range of 2.4-4.2 and measured at 616 nm,with apparent molar absorptivity of 1.84×104L·mol-1·cm-1,and in the range of (0.05-2.50)×10-5mol·L-1,when reacted in nearly neutral buffer medium of pH 4.5-7.5 and measured at 508 nm,with apparent molar absorptivity of 1.83×104L·mol-1·cm-1.A photometric method for determination of GTXC based on the above findings,in either slightly acidic medium or nearly neutral medium,was proposed.Three samples of GTXC injections were analyzed by the proposed method,giving results in conformity with the labelled values.Recovery tests were made by addition of GTXC standard solution to the injection samples,and values of recovery found were in the range of 98.5%-100.4%.
中图分类号 O657.31
所属栏目 工作简报
基金项目 重庆市教委科技基金(KJ051304)和教育部“春晖计划”(Z2005-1-55006)项目资助
收稿日期 2008/3/4
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备注江虹(1956-),女,四川资中人,教授,主要从事光谱分析研究.
引用该论文: JIANG Hong,LIU Jin-bo,LI Wei. Photometric Determination of Gatifloxacin by the Decoloration Reaction with Congo Red[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(4): 419~421
江虹,刘进波,李维. 刚果红褪色光度法测定加替沙星的含量[J]. 理化检验-化学分册, 2009, 45(4): 419~421
被引情况:
【1】廉淑芹,杨冬芝,郑笑笑,廉菁,王桂荣, "应用荧光光度法研究加替沙星与胃蛋白酶的相互作用",理化检验-化学分册 49, 253-255(2013)
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参考文献
【1】VISHWANATHAN K,BARTLETT M G,STEWART J T.Determination of gatifloxacin in human plasma by liquid chromatography/electrospray tandem mass spectrometry[J].Rapid Commun Mass Spectrom,2001,15(12):915-919.
【2】NABER C K,STEGHAFNER M.Concentrations of gatifloxacin in plasma and urine and penetration into prostatic and seminal fluid,ejaculate,and sperm cells after single oral administrations of 400 milligrams to volunteers[J].Antemicrob Agents Chemother,2001,45(1):293-297.
【3】刘志东,郭宏,宋成军,等.加替沙星眼用凝胶剂的HPLC测定[J].中国医药工业杂志,2005,36(9):570-571.
【4】李群力,蒋晓萌.加替沙星注射液的制备及HPLC法含量测定[J].中国抗生素杂志,2005,30(3):149-150,173.
【5】徐勤,秦雪莲,邓立东,等.HPLC法测定加替沙星胶囊的含量[J].药物分析杂志,2004,24(5):538-541.
【6】连宁,孙春燕,赵慧春.加替沙星的荧光光度法测定[J].分析测试学报,2002,21(1):79-81.
【7】朱斌,蒋受军,胡昌勤.高效毛细管电泳法测定加替沙星含量[J].药物分析杂志,2002,22(5):397-400.
【8】秦雪莲,徐勤,邓立东.紫外分光光度法测定加替沙星片的含量[J].西北药学杂志,2004,19(4):154-155.
【9】黄其春.紫外分光光度法测定加替沙星的含量[J].海峡药学,2002,14(2):34-35.
【10】孙涛,宋坤改,林赴田.盐酸加替沙星的药代动力学研究[J].中国新药杂志,2000,11(9):756-761.
【2】NABER C K,STEGHAFNER M.Concentrations of gatifloxacin in plasma and urine and penetration into prostatic and seminal fluid,ejaculate,and sperm cells after single oral administrations of 400 milligrams to volunteers[J].Antemicrob Agents Chemother,2001,45(1):293-297.
【3】刘志东,郭宏,宋成军,等.加替沙星眼用凝胶剂的HPLC测定[J].中国医药工业杂志,2005,36(9):570-571.
【4】李群力,蒋晓萌.加替沙星注射液的制备及HPLC法含量测定[J].中国抗生素杂志,2005,30(3):149-150,173.
【5】徐勤,秦雪莲,邓立东,等.HPLC法测定加替沙星胶囊的含量[J].药物分析杂志,2004,24(5):538-541.
【6】连宁,孙春燕,赵慧春.加替沙星的荧光光度法测定[J].分析测试学报,2002,21(1):79-81.
【7】朱斌,蒋受军,胡昌勤.高效毛细管电泳法测定加替沙星含量[J].药物分析杂志,2002,22(5):397-400.
【8】秦雪莲,徐勤,邓立东.紫外分光光度法测定加替沙星片的含量[J].西北药学杂志,2004,19(4):154-155.
【9】黄其春.紫外分光光度法测定加替沙星的含量[J].海峡药学,2002,14(2):34-35.
【10】孙涛,宋坤改,林赴田.盐酸加替沙星的药代动力学研究[J].中国新药杂志,2000,11(9):756-761.
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