基于氧化锌纳米棒的乙酰胆碱酯酶生物传感器用于辛硫磷农药测定
Determination of Phoxim Pesticides Using Acetylcholinesterase Biosensor Based on ZnO Nanorods
摘 要
以喷金的聚碳酸酯模板为工作电极,采用电沉积法从氯化锌和氯化钾溶液中制得氧化锌纳米棒.将沉积了氧化锌纳米棒的模板固定在打磨后的玻碳电极表面,并将模板溶解.再通过在氧化锌纳米棒修饰电极的表面直接固定乙酰胆碱酯酶,制备出乙酰胆碱酯酶生物传感器.自然晾干后,所得乙酰胆碱酯酶-氧化锌生物传感器用于辛硫磷农药的测定.试验结果表明：在含有0.5 mmol·L-1的巯基乙酰胆碱的pH 7.38磷酸盐缓冲溶液中,乙酰胆碱酯酶-氧化锌修饰电极的氧化峰电流显著提高,而再向其中加入酶抑制剂辛硫磷后,电流明显减小,在此基础上提出了一种高灵敏度的测定辛硫磷农药的方法.在优化的条件下,辛硫磷浓度在9.85×10-6~4.95×10-4mol·L-1之间,其浓度的对数与抑制率呈线性关系,检出限(3S/N)为5.99×10-6mol·L-1.
生物传感器
氧化锌纳米棒
辛硫磷
Acetylcholinesterase
Biosensor
ZnO nanorods
Phoxim
Abstract
Using gold-sprayed polycarbonate template as working electrode,ZnO nanorods were prepared by electro-deposition from a solution of ZnCl2 and KCl.The template deposited with ZnO nanorods was adhered and dissolved on surface of polished glassy carbon electrode (GCE).Finally,the acetylcholinesterase (AChE) biosensor was prepared by immobilizing the AchE directly onto the surface of the ZnO nanorods modified GCE.After air-drying,the biosensor,AChE-ZnO/GCE,was ready for use in determination of phoxim pesticides.It was found that in the presence of 0.5 mmol·L-1 hydrosulfoacetylcholine (ATCh),the oxidative peak current was remarkable raised at the AChE-ZnO/GCE in PBS of pH 7.38,but significant lowering of the peak current at the same biosensor,under same condition,was observed when phoxim was added.Linear relationship between the rate of decrease in peak current (I%) and logarithm of concentration of phoxim (a representative object for phoxime pesticides) was found in the range of 9.85×10-6-4.95×10-4mol·L-1.Detection limit (3S/N) of the method found was 5.99×10-6mol·L-1.
中图分类号 O657.1
所属栏目 工作简报
基金项目 云南省自然科学基金项目(No.2006B0028M)和云南省教育厅自然科学基金(No.07Z10087)
收稿日期 2008/2/4
修改稿日期
网络出版日期
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备注谭琳(1983-),女,重庆人,硕士研究生,从事电化学及生物传感器的研究.
引用该论文: TAN Lin,LU Xu-xiao,BAI Hui-ping,YANG Guang-ming,YANG Yun-hui. Determination of Phoxim Pesticides Using Acetylcholinesterase Biosensor Based on ZnO Nanorods[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(5): 567～570
谭琳,卢旭晓,白慧萍,杨光明,杨云慧. 基于氧化锌纳米棒的乙酰胆碱酯酶生物传感器用于辛硫磷农药测定[J]. 理化检验－化学分册, 2009, 45(5): 567～570
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参考文献
【1】SHULGA O,KIRCHHOFF J R.An acetylcholinesterase enzyme electrode stabilized by an electrodeposited gold nanoparticle layer[J].Electrochemistry Communications,2007,9(5):935．
【2】CHU Xia,DUAN Da-xue,SHEN Guo-li,et al.Amperometric glucose biosensor based on electrodeposition of platinum nanoparticles onto covalently immobilized carbon nanotube electrode[J].Talanta,2007,71(5):2040．
【3】YANG Ming-hui,YANG Yun-hui,LIU Yan-li,et al.Platinum nanoparticles-doped sol-gel/carbon nanotubes composite electrochemical sensors and biosensors[J].Biosensors and Bioelectronics,2006,21(7):1125．
【4】侯宪全,任湘菱,唐芳琼,等.纳米ZnO增强葡萄糖生物传感器的制备和应用[J].分析化学,2006,34(3):303．
【5】MAKOWER A,HALAMEK J,SKLADAL P,et al.New Principle of direct real-time monitoring of the interaction of cholinesterase and its inhibitors by piezoelectric biosensor[J].Biosens Bioelectron,2003,18(11):1329．
【6】朱青青,谢文,丁慧瑛.蜂蜜中多种有机磷农药残留量的气相色谱测定[J].理化检验-化学分册,2005,41(7):482．
【7】苟金萍,张艳,程淑华,等.蔬菜中有机磷和氨基甲酸酯类农药多残留联合检测方法[J].理化检验-化学分册,2005,41(12):939．
【8】王宗贤,高志贤,马成林.有机磷检测方法的研究进展[J].中国卫生检验杂志,2003,13(4):401．
【9】王艳,高志贤,戴树桂.农药残留免疫分析方法及其研究进展[J].农业环境科学学报,2003,22(1):125．
【10】AVRAMESCU A,ANDREESCU S,NOGUER T,et al.Biosensors designed for environmental and food quality control based on screen-printed graphite electrodes with different configurations[J].Analytical and Bioanalytical Chemistry,2002,374(1):25．
【11】康庆贺,杨长志.气相色谱法同时测定糙米中6种取代苯类及酰胺类农药残留[J].色谱,2003,21(4):421．
【12】NWOSU T N,PALLESCHI G,MASCINI M.Comparative studies of immobilized enzyme electrodes based on the inhibitory effect of nicotine on choline oxidase and acetylcholinesterase[J].Analytical Letters,1992,25(5):821．
【13】蒙绮芳,周锡良.测量农药和乙酰胆碱酯酶抑制物的光学式生物传感器[J].化学传感器,1996,16(3):192．
【2】CHU Xia,DUAN Da-xue,SHEN Guo-li,et al.Amperometric glucose biosensor based on electrodeposition of platinum nanoparticles onto covalently immobilized carbon nanotube electrode[J].Talanta,2007,71(5):2040．
【3】YANG Ming-hui,YANG Yun-hui,LIU Yan-li,et al.Platinum nanoparticles-doped sol-gel/carbon nanotubes composite electrochemical sensors and biosensors[J].Biosensors and Bioelectronics,2006,21(7):1125．
【4】侯宪全,任湘菱,唐芳琼,等.纳米ZnO增强葡萄糖生物传感器的制备和应用[J].分析化学,2006,34(3):303．
【5】MAKOWER A,HALAMEK J,SKLADAL P,et al.New Principle of direct real-time monitoring of the interaction of cholinesterase and its inhibitors by piezoelectric biosensor[J].Biosens Bioelectron,2003,18(11):1329．
【6】朱青青,谢文,丁慧瑛.蜂蜜中多种有机磷农药残留量的气相色谱测定[J].理化检验-化学分册,2005,41(7):482．
【7】苟金萍,张艳,程淑华,等.蔬菜中有机磷和氨基甲酸酯类农药多残留联合检测方法[J].理化检验-化学分册,2005,41(12):939．
【8】王宗贤,高志贤,马成林.有机磷检测方法的研究进展[J].中国卫生检验杂志,2003,13(4):401．
【9】王艳,高志贤,戴树桂.农药残留免疫分析方法及其研究进展[J].农业环境科学学报,2003,22(1):125．
【10】AVRAMESCU A,ANDREESCU S,NOGUER T,et al.Biosensors designed for environmental and food quality control based on screen-printed graphite electrodes with different configurations[J].Analytical and Bioanalytical Chemistry,2002,374(1):25．
【11】康庆贺,杨长志.气相色谱法同时测定糙米中6种取代苯类及酰胺类农药残留[J].色谱,2003,21(4):421．
【12】NWOSU T N,PALLESCHI G,MASCINI M.Comparative studies of immobilized enzyme electrodes based on the inhibitory effect of nicotine on choline oxidase and acetylcholinesterase[J].Analytical Letters,1992,25(5):821．
【13】蒙绮芳,周锡良.测量农药和乙酰胆碱酯酶抑制物的光学式生物传感器[J].化学传感器,1996,16(3):192．
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