提出了用高效液相色谱法测定奥硝唑阴道泡腾片中奥硝唑含量的方法.以Kromasil C18色谱柱(4.6 mm×250 mm,5 μm)为固定相,甲醇-水(30+70)溶液为流动相,在319 nm波长处用紫外分光检测器检测.奥硝唑的质量浓度在20.0~80.0 mg·L-1之间与其相应的峰面积值呈线性关系.应用此法测定奥硝唑阴道泡腾片中奥硝唑的含量,回收率在98.9%~100.1%之间,测定值的相对标准偏差(n=6)均小于0.9%.

HPLC was applied to the determination of ornidazole in ornidazole vaginal effervescent tablets.Kromasil C18 column (4.6 mm×250 mm,5 μm) was used as stationary phase,and methanol-water(30+70) solution was used as mobile phase in the HPLC analysis with UV detection at the wavelength of 319 nm.Linear relationship between values of peak area and mass concentration of ornidazole was obtained in the range of 20.0-80.0 mg·L-1.The proposed method was used in the determination of ornidazole in ornidazole vaginal effervescent tablets,values of recovery found by standard addition method were in the range of 98.9%-100.1%.Values of RSD′s (n=6) found were less than 0.9%.