羧甲基聚合-β-环糊精作为选择剂应用于毛细管电泳法拆分手性化合物
Use of Carboxymethyl-poly-β-cyclodextrin as Chiral Selective Agent in Capillary Electrophoretic Separation of Chiral Compounds
摘 要
提出了一种用毛细管电泳拆分3种未衍生化的氨基酸对映体(苯丙氨酸,酪氨酸,色氨酸)和一种手性药物(芬氟拉明)的方法.以水溶性羧甲基聚合-β-环糊精为手性选择剂,采用毛细管区带电泳模式,考察了手性选择剂浓度、缓冲溶液pH值、柱温及电压对分离的影响.4种手性化合物在各自优化的试验条件下,均达到基线分离.此法操作简单,可用于这4种手性化合物的质量控制.
羧甲基聚合-β-环糊精
氨基酸
手性药物
芬氟拉明
Capillary electrophoresis
Carboxymethyl-poly-β-CD
Amino acids
Chiral drug
Fenfluramine
Abstract
Capillary electrophoresis (CE) was applied to enantiomorphous separation of 3 un-derivatized amino acids,i.e.,phenylalanine,tyrosin,tryptophan,and a chiral drug,“Fenfluramine”,and the water soluble carboxymethyl-poly-β-cyclodextrin (CM-poly-β-CD) was used as chiral selective agent.Under the mode of zone electrophoresis,conditions for the separation,including the concentration of CM-poly-β-CD,pH-value of buffer solution,temperature and pressure of the column were tested individually and optimized.Under the respective optimum condition,enantiomorphous separation of each of the 4 chiral compounds was attained and clear separation from the baseline was observed.The proposed method was found feasible to be applied to quality control of the 4 chiral compounds.
中图分类号 O657.8
所属栏目 工作简报
基金项目 黑龙江省科技厅攻关项目(GC02A313-1)
收稿日期 2008/2/24
修改稿日期
网络出版日期
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备注李英杰(1961-),男,齐齐哈尔人,教授,主要从事色谱分析和光谱分析研究.
引用该论文: LI Ying-jie,CHEN Zhi-chun,GAO Li-di,CHEN Zhen-quan. Use of Carboxymethyl-poly-β-cyclodextrin as Chiral Selective Agent in Capillary Electrophoretic Separation of Chiral Compounds[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(5): 586～588
李英杰,陈治春,高立娣,陈振泉. 羧甲基聚合-β-环糊精作为选择剂应用于毛细管电泳法拆分手性化合物[J]. 理化检验－化学分册, 2009, 45(5): 586～588
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参考文献
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【10】FANALI S J.Enantioselective determination by capillary electrophoresis with cyclodextrins as chiral selectors[J].J Chromatogr A,2000,875(1/2):89-122．
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【12】李英杰,高立娣.水溶性β-CD聚合物在毛细管电泳中分离氨基酸对映体[J].分析试验室,2005,24(12):50-53．
【13】WREN A C,ROWE C R.Theoretical aspects of chiral separation in capillary electrophoresis[J].J Chromatogr A,1992,603(1/2):235-241．
【2】ZHENG Zhi-xia,QU Feng,LIN Jin-ming.Investigation of mixed chiral selectors of different metal ion-L-alanine complex and β-cyclodextrin on the chiral separation of dansyl amino acids with capillary electrophoresis[J].Chinense Journal of Chemistry,2003,21(11):1478-1484．
【3】卢小宁,陈义.基于二元手性选择剂协同作用的毛细管电泳拆分氨基酸对映体研究[J].高等学校化学学报,2002,23(5):822-824．
【4】张莹,张阳阳,杨奇,等.氨基酸对映体拆分的研究进展[J].辽宁大学学报,2002,29(3):193-196．
【5】YANG Li-li,YUAN Zhuo-bin.Comparisiong of enantioseparation of amino acid derivatives in aqueous and mixed aqueous-organic media by capilly zone electrophoresis[J].Fresenius J Anal Chem,1999,365:541-544．
【6】周权,姚彤炜,曾苏.手性药物的相互作用[J].中国药学杂志,1999,34(11):721-724．
【7】张京芳,吕宪祥,刘志民.芬氟拉明的一般药理及毒理作用[J].中国药物依赖性杂志,2002,11(4):316-318．
【8】李继革,胡小刚.毛细管电泳技术及其在药物分析中的应用[J].广州化工,2000,28(2):25-28．
【9】邓延倬,何金兰.毛细管电泳技术及其应用[M].北京:科学出版社,1996:318．
【10】FANALI S J.Enantioselective determination by capillary electrophoresis with cyclodextrins as chiral selectors[J].J Chromatogr A,2000,875(1/2):89-122．
【11】李雪岩.环糊精类气相色谱固定相和毛细管电泳手性选择剂的合成及应用研究[D].北京:北京理工大学,2000:84-85．
【12】李英杰,高立娣.水溶性β-CD聚合物在毛细管电泳中分离氨基酸对映体[J].分析试验室,2005,24(12):50-53．
【13】WREN A C,ROWE C R.Theoretical aspects of chiral separation in capillary electrophoresis[J].J Chromatogr A,1992,603(1/2):235-241．
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