提出了超高效液相色谱法同时测定洗发类化妆品中3种去屑剂(水杨酸、酮康唑、氯咪巴唑)含量的方法.以ACQUITY UPLC-BEH C18色谱柱 (2.1 mm×100 mm,1.7 μm)为固定相及乙腈-甲醇及pH 4.0磷酸盐-EDTA缓冲溶液(57.5+8.5+34)混合溶液为流动相,在230 nm波长处用二极管阵列检测器检测.3种去屑剂水杨酸、酮康唑、氯咪巴唑的质量浓度与其相应的峰面积值呈线性关系,线性范围分别为50~500,50~500,100~1 000 mg·L-1.应用此法测定洗发类化妆品中3种去屑剂的含量,回收率在94.2%~99.4%之间,相对标准偏差(n=6)均小于0.35%.

Amounts of 3 anti-dandruff agents in shampoo cosmetics,including salicylic acid,ketoclonazole and climbazole,were simultaneously determined by UPLC.ACQUITY UPLC-BEH C18 column (2.1 mm×100 mm,1.7 μm) was used as stationary phase,and mixture contaning acetonitrile methanol and phosphate-EDTA buffer solution of pH 4 (57.5+8.5+34) was used as mobile phase in the UPLC with DAD at the wavelength of 230 nm.Linear relationships between values of peak areas and mass concentrations of 3 anti-dandruff agents were obtained,with linear ranges of 50-500 mg·L-1 (for salicylic acid),50-500 mg·L-1 (for ketoclonazole),and 100-1 000 mg·L-1 (for climbazole).The proposed method was used in the determination of 3 anti-dandruff agents in shampoo cosmetics,values of recovery found by standard addition method were in the range of 94.2%-99.4%,with values of RSD′s (n=6) less than 0.35%.