荧光光度法测定槐花中槲皮素
Fluorophotometric Determination of Quercetin in Flossophorae
摘 要
研究了基于槲皮素和铝的络合反应来测定槲皮素的荧光光度法,试验结果表明:铝与槲皮素的配合物在pH 9.15的BR缓冲溶液中进一步与BO3-3形成三元体系,在452 nm激发波长下,于516 nm发射波长处测定其荧光强度,槲皮素浓度在1.0×10-7~6.0×10-6mol·L-1范围内配合物的荧光强度与槲皮素的浓度呈线性关系,检出限(3S/N)为2.1×10-7mol·L-1.该方法用于槐花提取物中槲皮素的测定,并以实样为基体用标准加入法做了回收试验,测得回收率在101.5%~112.5%之间.
铝
槲皮素
槐花
Fluorophotometric
Aluminum(Ⅲ)
Quercetin
Flossophorae
Abstract
In a B-R buffer medium of pH 9.15,a ternary complex was formed among Al(Ⅲ),quercetin and borate ion (BO3-3),and its fluorescence property was measured at the wavelengths of (λex) 452 nm and (λem) 516 nm.Linear relationship between values of fluorescence intensity and concentration of quercetin was kept in the range from 1.0×10-7-6.0×10-6mol·L-1.Detection limit (3S/N) of the reaction found was 2.1×10-7mol·L-1.Samples of flossophorae extract were analyzed by the proposed method,and test for recovery was made on these samples as matrixes,giving values of recovery in the range of 101.5%-112.5%.
中图分类号 O657.31
所属栏目 工作简报
基金项目 廊坊师范学院科学研究项目(LSEYE 200309)
收稿日期 2008/3/19
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备注苏文斌(1978-),男,安徽桐城人,讲师,硕士,研究方向为发光分析和环境分析.
引用该论文: SU Wen-Bin,LAN Rui-Jia. Fluorophotometric Determination of Quercetin in Flossophorae[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(6): 704～706
苏文斌,兰瑞家. 荧光光度法测定槐花中槲皮素[J]. 理化检验－化学分册, 2009, 45(6): 704～706
被引情况:
【1】卞惠芳,魏杰,王涛,张钱丽, "碳纳米管-石墨烯修饰电极测定洋葱中槲皮素的含量",理化检验－化学分册 52, 1246-1250(2016)
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参考文献
【1】许进军,何东初.槲皮素研究进展[J].实用预防医学,2006,13(4):1095-1097．
【2】李满秀,张静,张海容,等.等吸收紫外光度法同时测定槐米中的芦丁和槲皮素[J].光谱实验室,2005,22(1):42-45．
【3】周漩,宋粉云,钟兆键.薄层色谱扫描法测定槐米中的芦丁和槲皮素[J].中国实验方剂学杂志,2006,18(8):14-16．
【4】SWAROOP A,GUPTA A P,SINHA A K.Simultaneous determination of quercetin,rutin and coumaric acid in flowers of Rhododendron arboreum by HPTLC[J].Chromatographia,2005,62(11/12):649-652．
【5】徐雄良,张志荣,柯尊洪,等.RP-HPLC法测定槐米中槲皮素的含量[J].中草药,2003,34(6):565-567．
【6】JIN D,HAKAMATA H,TAKAHASHI K,et al.Determination of quercetin in human plasma after ingestion of commercial canned green tea by semi-micro HPLC with electrochemical detection[J].Biomedical Chromatography,2004,18(9):662-666．
【7】YAN Jian-wei,WANG Miao,LU Jian-de.Determination of rutin,quercetin,and chlorogenic acid in mulberry leaves by capillary zone electrophoresis[J].Analytical Letters,2004,37(15):3287-3297．
【8】刘松青,穆海川,陈永川.高效毛细管电泳法测定槐花和槐角中芦丁的含量[J].华西药学杂志,2002,17(4):284-285．
【9】XU G R,KIM S.Selective determination of quercetin using carbon nanotube-modified electrodes[J].Electroanalysis,2006,18(18):1786-1792．
【10】冯瑞琴,沈霞,丁芬.槲皮素-过氧化氢荧光体系的研究及应用[J].北京师范大学学报:自然科学版,2004,40(4):549-501．
【11】国家药典委员会.中华人民共和国药典:一部[M].北京:化学工业出版社,2005:246-247．
【2】李满秀,张静,张海容,等.等吸收紫外光度法同时测定槐米中的芦丁和槲皮素[J].光谱实验室,2005,22(1):42-45．
【3】周漩,宋粉云,钟兆键.薄层色谱扫描法测定槐米中的芦丁和槲皮素[J].中国实验方剂学杂志,2006,18(8):14-16．
【4】SWAROOP A,GUPTA A P,SINHA A K.Simultaneous determination of quercetin,rutin and coumaric acid in flowers of Rhododendron arboreum by HPTLC[J].Chromatographia,2005,62(11/12):649-652．
【5】徐雄良,张志荣,柯尊洪,等.RP-HPLC法测定槐米中槲皮素的含量[J].中草药,2003,34(6):565-567．
【6】JIN D,HAKAMATA H,TAKAHASHI K,et al.Determination of quercetin in human plasma after ingestion of commercial canned green tea by semi-micro HPLC with electrochemical detection[J].Biomedical Chromatography,2004,18(9):662-666．
【7】YAN Jian-wei,WANG Miao,LU Jian-de.Determination of rutin,quercetin,and chlorogenic acid in mulberry leaves by capillary zone electrophoresis[J].Analytical Letters,2004,37(15):3287-3297．
【8】刘松青,穆海川,陈永川.高效毛细管电泳法测定槐花和槐角中芦丁的含量[J].华西药学杂志,2002,17(4):284-285．
【9】XU G R,KIM S.Selective determination of quercetin using carbon nanotube-modified electrodes[J].Electroanalysis,2006,18(18):1786-1792．
【10】冯瑞琴,沈霞,丁芬.槲皮素-过氧化氢荧光体系的研究及应用[J].北京师范大学学报:自然科学版,2004,40(4):549-501．
【11】国家药典委员会.中华人民共和国药典:一部[M].北京:化学工业出版社,2005:246-247．
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