Chiral Separation of Enantiomers of Quinolone Drugs by Capillary Electrophoresis
摘 要
采用毛细管电泳法,以铜(Ⅱ)-L-异白氨酸为手性拆分剂,同时分离了氧氟沙星、洛美沙星、司帕沙星和加替沙星四种喹诺酮类药物对映体.考察了手性拆分剂的种类、配比和浓度,缓冲溶液的种类、浓度和pH值,有机添加剂的种类和用量,分离电压等试验条件对分离效果的影响.含8 mmol·L-1 L-异白氨酸和4 mmol·L-1 硫酸铜的pH 8.5的30 mmol·L-1 硼酸钠-盐酸缓冲溶液中,氧氟沙星和加替沙星对映体实现分离;在含20 mmol·L-1 L-异白氨酸,10 mmol·L-1硫酸铜和乙腈(5+95)的pH 9.0的20 mmol·L-1的Tris-硼酸钠缓冲溶液中,司帕沙星、洛美沙星和加替沙星对映体同时实现完全分离.
Abstract
Separation of enantiomers of 4 quinolones (ofloxacin,lomefloxacin,sparfloxacin,and gatifloxacin) were preformed simultaneously by capillary electrophoresis with copper(Ⅱ)-L-isoleucine as chiral resolving agent.Various factors influential to the efficiency of separation,including kinds of chiral resolving agent,ratio and concentration of chiral resolving agent,kinds of buffer solutions,pH and concentration of buffer solution,kinds and amount of organic additives,were studied and optimized.The enantiomers of ofloxacin and gatifloxacin were completely separated in 30 mmol·L-1 Na2B4O7-HCl buffer solution of pH 8.5 containing 8 mmol·L-1 L-isoleucine solution and 4 mmol·L-1 CuSO4 solution,the enantiomers of omefloxacin,sparfloxacin,and gatifloxacin were completely separated in 20 mmol·L-1 Tris-Na2B4O7 buffer solution of pH 9.0 containing 20 mmol·L-1 L-isoleucine solution,10 mmol·L-1 CuSO4 solution with addition of 5% by volume of acetonitrile.
中图分类号 O657.8
所属栏目 工作简报
基金项目 广西自然科学资金资助项目(0640038);肇庆市科技计划资助项目(10560),肇庆学院自然科学基金资助项目(0737)
收稿日期 2008/5/6
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备注韦寿莲(1965-),女,广西贵港人,教授,研究方向为药物分析.
引用该论文: WEI Shou-lian,YAN Zi-jun,Qi Li-hua. Chiral Separation of Enantiomers of Quinolone Drugs by Capillary Electrophoresis[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(6): 714~716
韦寿莲,严子军,戚利华. 毛细管电泳手性拆分喹诺酮类药物对映体[J]. 理化检验-化学分册, 2009, 45(6): 714~716
被引情况:
【1】唐晓丹,张燕,贾宏敏,丛景香,田雨, "洛美沙星对映体在Chiralcel OJ柱上的拆分机理",理化检验-化学分册 47, 764-767(2011)
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参考文献
【1】邓延倬,何金兰.高效毛细管电泳[M].北京:科学出版社,1996:33-34.
【2】ZHU Xiao-feng,DING Yong-sheng,LIN Bing-cheng,et al.Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis[J].Electrophoresis,1999,20(9):1869-1877.
【3】DE-BOER T,MOL R,DE-ZEEUW R A,et al.Enantioseparation of Ofloxacin in urine by capillary electrokinetic choromatography using charged cyclodextrins as chiral selectors and assessment of enantioconverion[J].Electrophoresis,2001,22(7):1413-1418.
【4】俞犄,王义明,罗国安.氧氟沙星对映体的毛细管电泳手性分离[J].药学学报,1997,32(3):203-206.
【5】丁文静,许旭,王尔华,等.帕苏沙星的毛细管电泳手性分离[J].色谱,2004,22(2):192-192.
【6】CHO S I,LEE K N,KIN Y K,et al.Chiral separation of gemifloxacin in sodium containing media using chiral crown ether as a chiral selector by capillary and microchip electrophoresis[J].Electrophoresis,2002,23(6):972-977.
【7】YAN Hong-yuan,ROW K H.Rapid chiral separation and impurity determination of levofloxacin by ligand-exchange chromatography[J].Anal Chim Acta,2007,584(1):160-165.
【8】CHIN Zi-lin,UCHIYAMA K,HOBO T.Chiral resolution of dansyl amino acids by ligand exchange-capillary electrophoresis using Cu(Ⅱ)-L-prolinamides as chiral selector[J].Anal Chim Acta,2004,523(1):1-7.
【2】ZHU Xiao-feng,DING Yong-sheng,LIN Bing-cheng,et al.Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis[J].Electrophoresis,1999,20(9):1869-1877.
【3】DE-BOER T,MOL R,DE-ZEEUW R A,et al.Enantioseparation of Ofloxacin in urine by capillary electrokinetic choromatography using charged cyclodextrins as chiral selectors and assessment of enantioconverion[J].Electrophoresis,2001,22(7):1413-1418.
【4】俞犄,王义明,罗国安.氧氟沙星对映体的毛细管电泳手性分离[J].药学学报,1997,32(3):203-206.
【5】丁文静,许旭,王尔华,等.帕苏沙星的毛细管电泳手性分离[J].色谱,2004,22(2):192-192.
【6】CHO S I,LEE K N,KIN Y K,et al.Chiral separation of gemifloxacin in sodium containing media using chiral crown ether as a chiral selector by capillary and microchip electrophoresis[J].Electrophoresis,2002,23(6):972-977.
【7】YAN Hong-yuan,ROW K H.Rapid chiral separation and impurity determination of levofloxacin by ligand-exchange chromatography[J].Anal Chim Acta,2007,584(1):160-165.
【8】CHIN Zi-lin,UCHIYAMA K,HOBO T.Chiral resolution of dansyl amino acids by ligand exchange-capillary electrophoresis using Cu(Ⅱ)-L-prolinamides as chiral selector[J].Anal Chim Acta,2004,523(1):1-7.
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