Simultaneous Determination of Vitamin B6,Nicotinamide and Calcium Pentothenate by RP-HPLC
摘 要
应用反相高效液相色谱法同时测定了维生素营养品中维生素B6、烟酰胺和泛酸钙.样品用由磷酸盐缓冲溶液(pH 3.0)和甲醇组成的提取液超声提取5 min后,再置于65~70 ℃的水浴中加热10 min,所得提取液供高效液相色谱法分析用.色谱分析中用Inertsil pH-3苯基色谱柱作固定相,用由0.05 mol·L-1磷酸二氢钾、甲醇及乙腈以90比6比4的体积比混合的溶液为流动相,并在210 nm波长处进行紫外检测.以测得的峰面积值对上述3种化合物的浓度制作标准曲线,其线性关系通过回归分析予以检验,3个回归方程的相关系数均大于0.999 9.以实样为基体,用标准加入法对所提出的方法进行了回收试验,测得上述3种化合物的回收率依次为97.4%,102.4%及100.3%.
Abstract
The method of RP-HPLC was applied to simultaneous determination of vitamin B6,nicotinamide and calcium pentothenate in some vitamin nutriments. The sample was extracted ultrasonically with an extractant containing phosphate buffer solution (pH 3.0) and methanol for 5 min and then heated at 65-70 ℃ in a water bath for 10 min. The extract obtained was used for the HPLC analysis. The Inertsil pH-3 Phenyl column was used as stationary phase,and a mixed solution of 0.05 mol·L-1 KH2PO4,methanol and acetonitrile mixed in the ratio of 90 to 6 to 4 by volume,was used as mobile phase. UV detection was made at the wavelength of 210 nm. Linearity of the standard curves between the values of peak area and concentration of the 3 compounds was checked by regression analysis,giving values of correlation coefficients greater than 0.999 9. Test for recovery was made by standard addition method with a substantial sample as the matrix,the results of recovery were found to be 97.4% for vitamin B6,100.3% for calcium pentothenate and 102.4% for nicotinamide.
中图分类号 O652.63
所属栏目 工作简报
基金项目 广西壮族自治区卫生厅自筹经费立项课题(Z2006307)
收稿日期 2008/4/21
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备注李少旦(1975-),男,湖南人,主管技师,硕士,主要研究方向为仪器分析.
引用该论文: LI Shao-dan,PENG Wei-fang. Simultaneous Determination of Vitamin B6,Nicotinamide and Calcium Pentothenate by RP-HPLC[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(7): 800~802
李少旦,彭卫芳. 反相高效液相色谱法同时测定维生素B6、烟酰胺和泛酸钙[J]. 理化检验-化学分册, 2009, 45(7): 800~802
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【2】刘莉治,罗浇燕,周洪伟,等.快速测定食品中烟酰胺、维生素B1、B6的含量[J].中国卫生检验杂志,2005,15(6):730-731.
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【4】隋玉荣,张莉,黄志东,等.离子对色谱法测定复方叶酸片中维生素B6、B12及叶酸的含量[J].药物分析杂志,2003,23(4):291-293.
【5】邓富良,陈本美,陈新,等.高效液相色谱法测定维康胶囊中硫胺素、核黄素、盐酸吡多辛及烟酰胺[J].中国药事,2005,19(4):231-233.
【6】申烨华,张萍,孔祥虹,等.高效液相色谱法同时测定扁桃仁中的水溶性维生素C,B1,B2和B6[J].色谱,2005,23(5):538-541.
【7】蒋晔,刘菊红,郝晓花.反相高效液相色谱法同时测定9种水溶性维生素[J].药物分析杂志,2005,25(3):339-341.
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