Sm(Ⅲ)(CAI)3与DNA相互作用的反应机理
Mechanism of Interaction of the Coordination Complex Sm(Ⅲ)(CAI)3 with DNA
摘 要
应用吸收光谱、荧光光谱和粘度法并以吖啶橙作探针研究了Sm(Ⅲ)(CAI)3与DNA的相互作用.研究表明,钙黄绿素(CAI)与钐(Ⅲ)形成了配合物,其结合比n钐(Ⅲ)∶nCAI=1∶3,生成八面体构型稀土配合物,Sm(Ⅲ)(CAI)3与DNA的结合常数K=2.09×104L·mol-1.Scatchard方程表明该配合物与DNA的结合为中等强度的嵌插和部分静电作用.
Sm(Ⅲ)(CAI)3
DNA
吖啶橙
作用机理
荧光光度法
吸收光度法
Coordination complex
Sm(Ⅲ)(CAI)3
DNA
Acridine orange
Reaction mechanism
Fluorophotometry
Spectrophotometry
Abstract
Mechanism of the interaction of the coordination complex of samarium(Ⅲ) and calcein (CAI in short) with DNA was studied by spectrophotometry,fluorophotometry and viscositometry using acridine orange (AO in short) as probe.As shown by experimental results,the coordination complex formed between Sm(Ⅲ) and CAI has an octahedronal molecular structure with its binding mole ratio (nSm(Ⅲ)∶nCAI) of 1 to 3,and the Sm(Ⅲ)-CAI complex reacts with DNA to form an association complex with the combination constant (K) of 2.09×104L·mol-1.As shown by ploting according to the Scatchard equation,the characteristics of the complex between Sm(Ⅲ)-CAI and DNA belongs to types of intercalation of middle strength and partial electrostatic.
中图分类号 O657.32
所属栏目 工作简报
基金项目 国家自然科学基金项目(No.30572254)
收稿日期 2008/5/7
修改稿日期
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备注拓宏桂(1956-),男,陕西子洲人,教授,研究方向为分析化学及化学教学.
引用该论文: TA Hong-gui,LI Hong-bo,WANG Xing-ming,HU Zhi-biao,SONG Hong-tao,ZHAO Jin-hua,DING Li-sheng. Mechanism of Interaction of the Coordination Complex Sm(Ⅲ)(CAI)3 with DNA[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(8): 952～956
拓宏桂,黎泓波,王兴明,胡志彪,宋宏涛,赵金花,丁立生. Sm(Ⅲ)(CAI)3与DNA相互作用的反应机理[J]. 理化检验－化学分册, 2009, 45(8): 952～956
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【8】王兴明,黎泓波,刘海萍,等.甲基百里酚蓝-钐(Ⅲ)配合物与鲱鱼精DNA的相互作用[J].化学学报,2006,64(20):2115-2119．
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【17】韩高义,杨频.四[对-(羧基甲氧基)苯基]卟啉金属配合物(M=Zn,Cu,Ni)的π-π配合及其二丁基锡(Ⅳ)酯衍生物的抗癌活性[J].高等学校化学学报,2001,22(12):1993-1995．
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