基于分子印迹识别的化学发光微流控传感器芯片测定双嘧达莫
Chemiluminescence Determination of Dipyridamole with Microfluidic Sensor Chip Based on Molecular Imprinted Recognition
摘 要
试验中,以双嘧达莫为模板分子,α-甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,氯仿为溶剂,合成了双嘧达莫分子印迹聚合物.将此聚合物填充在长8 mm,宽1 mm,深0.5 mm的微流控芯片检测池中作为分子识别物质,设计了一种新型的化学发光微流控传感器芯片测定双嘧达莫.双嘧达莫被此聚合物在线吸附并识别,被吸附的双嘧达莫与鲁米诺和铁氰化钾混合溶液反应并导致其化学发光强度增大.该传感器对双嘧达莫响应范围为1.0~20 μg·L-1,检出限(3σ)为0.5 μg·L-1,对10 μg·L-1双嘧达莫连续平行测定7次,其相对标准偏差为4.6%.
分子印迹识别
化学发光
双嘧达莫
Microfluidic sensor chip
Molecular-imprinted recognition
Chemiluminescence
Dipyridamole
Abstract
A new chemiluminescence microfluidic sensor chip based on molecular-imprinted recognition,used for determination of dipyridamole was designed and fabricated.The molecular imprinted polymer was synthesized by using dipyridamol as template molecule,α-methyl propenoic acid as functional monomer and ethylene glycol di-α-methyl propenoate as cross-linking agent and with chloroform as solvent.The polymer obtained was filled into the detection cell of the microfluidic sensor chip,with its dimension of 8 mm(length)×1 mm(width)×0.5 mm(depth),acting as recognition element.Dipyridamole was on-line adsorbed and recognized by the molecular-imprinted polymer of the sensor chip,and the adsorbed dipyridamole was reacted with a mixed solution of luminol and potassium ferricyanide,resulting an enhancement of chemiluminence (CL) signal.The magnitude of increase of CL intensity kept a linear relationship with mass concentration of dipyridamole in the range of 1.0-20 μg·L-1.Detection limit (3σ) of the method was found to be 0.5 μg·L-1.Test for precision was made at the concentration level of 10 μg·L-1 of dipyridamole,and value of RSD (n=7) found was 4.6%.
中图分类号 O657
所属栏目 试验与研究
基金项目 国家自然科学基金(30470886),江西省教育厅科技项目(GJJ08413)
收稿日期 2008/3/15
修改稿日期
网络出版日期
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备注何德勇(1978-),男,四川大竹人,副教授,博士,研究方向为化学发光及微流控芯片.
引用该论文: HE De-yong,ZHANG Zhu-jun,HE Shu-hua. Chemiluminescence Determination of Dipyridamole with Microfluidic Sensor Chip Based on Molecular Imprinted Recognition[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(9): 1025～1028
何德勇,章竹君,何树华. 基于分子印迹识别的化学发光微流控传感器芯片测定双嘧达莫[J]. 理化检验－化学分册, 2009, 45(9): 1025～1028
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【7】GREENE N T,SHIMIZU K D.Colorimetric molecularly imprinted polymer sensor array using dyedisplacement[J].J Am Chem Soc,2005,127:5695．
【8】PULGARN J A M,MOKINA A A,LPEZ P F.Simultaneous determination of atenolol,propranolol,dipyridamole and amiloride by means of non-linear variable-angle synchronous fluorescence spectrometry[J].Anal Chim Acta,1998,370:9．
【9】SHOUKRY A F,ABDEL G N T,ISSA Y M.Spectrophotometric determination of dipyridamole and chlorpheniramine maleate using some chromtropic acid azo dyes[J].Anal Lett,2001,34:1689．
【10】周莉,平其能,杨霖.HPLC法测定复方制剂中双嘧达莫、阿司匹林及水杨酸限量检测[J].2003,3:199-201．
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【12】何树华,吕弋,何德勇,等.鲁米诺铁氰化钾化学发光体系测定双嘧达莫[J].分析试验室,2004,23:5．
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【16】刘清慧,吕九如,冯娜.铁氰化钾-鲁米诺体系后化学发光反应及其分析应用研究-分子印迹-后化学发光法测定双嘧达莫[J].高等学校化学学报,2006,27(6):1036．
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