应用三维荧光技术,结合化学计量学中的二阶校正算法,在诺氟沙星和左氧氟沙星荧光光谱严重重叠,以及干扰物质存在下对血浆中两种喹诺酮类药物进行了同时定量分析.三维荧光激发波长范围265~510 nm,发射波长范围300~650 nm.交替不对称三线性分解(AATLD)算法解析得到的诺氟沙星和左氧氟沙星的平均回收率分别为96.9%和103.9%.方法前处理简单、不需预先分离、可以快速定量分析血浆中光谱相互干扰的待测药物的含量.

Simultaneous determination of norfloxacin and levofloxacin by fluorospectrophotometry with scanning in the excitation wavelength range of 265-510 nm and in the emission wavelength range of 300-650 nm. Data of fluorescence intensity measured were treated by the algorithms of 2nd order calibration and 3-dimensional data array analysis. As analyzed by the algorithm of AATLD,values of average recovery of 96.9% (for norfloxacin) and 103.9% (for levofloxacin) were found. It was shown by the analytical results of the method proposed,the interferences due to overlapping of fluorescence spectra of the 2 analytes and also those unknown co-existing substances present in the sample,were effectively eliminated. The present method was proved to be simple and rapid for analysis of plasma samples without the necessity of pre-separation.