超高效液相色谱-串联质谱法快速测定香精中香豆素的含量
UHPLC-MS/MS Determination of Coumarin in Essence
摘 要
香精经乙醇提取,采用超高效液相色谱-串联质谱快速测定了香精中香豆素的含量.以ACQUITYTM BEH C18分离柱,以乙腈和乙酸为流动相梯度洗脱,采用多反应监测正离子模式检测,定量离子对为m/z 147.2,65.0,定性离子对为m/z 147.2,91.0.线性范围为20~8 000 μg·L-1,检出限(3S/N)为10 μg·L-1.加标回收率在96.6%~101.0%之间,相对标准偏差(n=5)为3.9%~5.7%.
香豆素
香精
UPLC-MS/MS
Essence
Coumarin
Abstract
The sample of essence was extracted with ethanol,and the solution obtained was determined rapidly by UPLC. The ACQUITYTM BEH C18 column was used as stationary phase;and a mixture of acetonitrile and acetic acid was used as mobile phase;positive electrospray ionization as well as multiple reaction monitoring mode was used in the detection. Ion pair used for qualitative analysis was: m/z 147.2,65.0,ion pair use for quantitative analysis was m/z 147.2,91.0. Linearity range was found between 20-8 000 μg·L-1,and the detection limit (3S/N) found was 10 μg·L-1. Values of recovery found by addition of standard coumarin were found in the range of 96.6%-101.0%,and values of RSD (n=5) was in the range of 3.9%-5.7%.
中图分类号 O657.7
所属栏目 工作简报
基金项目
收稿日期 2009/9/2
修改稿日期
网络出版日期
作者单位点击查看
备注吴新华(1984-),男,湖北监利人,硕士,主要研究方向为烟草化学.
引用该论文: WU Xin-hua,ZHU Rui-zhi,LU She-ming,WANG Kai,MENG Zhao-yu,MU Ding-rong,MIAO Ming-ming. UHPLC-MS/MS Determination of Coumarin in Essence[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2009, 45(12): 1407～1409
吴新华,朱瑞芝,陆舍铭,王凯,孟昭宇,牟定荣,缪明明. 超高效液相色谱-串联质谱法快速测定香精中香豆素的含量[J]. 理化检验－化学分册, 2009, 45(12): 1407～1409
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】凡实.食品添加剂的使用与管理[J].肉类研究,2003,4:30-33．
【2】李洁,李楠,武婷,等.气相色谱质谱法测定化妆品中三种香豆素类物质的含量[J].生命科学仪器,2006,4:33-36．
【3】肖上甲,王世平.气相色谱法测定化妆品中6-甲基香豆素[J].环境与健康杂志,2002,19(2):146-147．
【4】邓捷圆,高广慧,赵春杰,等.HPLC法同时测定不同产地白芷中2种香豆素的含量[J].沈阳药大学学报,2004,21(5):354-357．
【5】张兴福,薛艳,张铁军,等.HPLC法测定草木犀中香豆素[J].中草药,2005,36(11):1122-1124．
【6】高秋涛.HPLC法测定甘草附子汤中甘草苷和香豆素的含量[J].沈阳药科大学学报,2003,20(6):439-441．
【7】YU K,LITTLE D,PLUMB R,et al. High-throughput quantification for a drug mixture in rat plasma-a comparison of Ultra Performance (TM) liquid chromatography/tandem mass spectrometry with high-performance liquid chromatography/tandem mass spectrometry[J]. Rapid Communication in Mass Spectrometry,2006,20:544-552．
【8】LI Xiao-qin,XIONG Zhi-li,YING Xi-xiang,et al. A rapid ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric method for the qualitative and quantitative analysis of the constituents of the flower of Trollius ledibouri Reichb[J]. Analytica Chimica Acta,2006,580:170-180．
【9】HUERTA-FONTELA M,GALCERAN M T,VENTURA F. Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Analysis of Stimulatory Drugs of Abuse in Wastewater and Surface Waters[J]. Anal Chem,2007,79:3821-3829．
【10】JACOB S S,SMITH N W,LEGIDO-QUIGLEY C. Assessment of Chinese medicinal herb metabolite profiles by UPLC-MS-based methodology for the detection of aristolochic acids[J]. Journal of Separation Science,2007,30:1200-1206．
【11】MATUSZEWSKI B K,CONSTANZER M L,CHAVEZ-ENG C M. Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS[J]. Anal Chem,2003,75:3019-3030．
【2】李洁,李楠,武婷,等.气相色谱质谱法测定化妆品中三种香豆素类物质的含量[J].生命科学仪器,2006,4:33-36．
【3】肖上甲,王世平.气相色谱法测定化妆品中6-甲基香豆素[J].环境与健康杂志,2002,19(2):146-147．
【4】邓捷圆,高广慧,赵春杰,等.HPLC法同时测定不同产地白芷中2种香豆素的含量[J].沈阳药大学学报,2004,21(5):354-357．
【5】张兴福,薛艳,张铁军,等.HPLC法测定草木犀中香豆素[J].中草药,2005,36(11):1122-1124．
【6】高秋涛.HPLC法测定甘草附子汤中甘草苷和香豆素的含量[J].沈阳药科大学学报,2003,20(6):439-441．
【7】YU K,LITTLE D,PLUMB R,et al. High-throughput quantification for a drug mixture in rat plasma-a comparison of Ultra Performance (TM) liquid chromatography/tandem mass spectrometry with high-performance liquid chromatography/tandem mass spectrometry[J]. Rapid Communication in Mass Spectrometry,2006,20:544-552．
【8】LI Xiao-qin,XIONG Zhi-li,YING Xi-xiang,et al. A rapid ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric method for the qualitative and quantitative analysis of the constituents of the flower of Trollius ledibouri Reichb[J]. Analytica Chimica Acta,2006,580:170-180．
【9】HUERTA-FONTELA M,GALCERAN M T,VENTURA F. Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry Analysis of Stimulatory Drugs of Abuse in Wastewater and Surface Waters[J]. Anal Chem,2007,79:3821-3829．
【10】JACOB S S,SMITH N W,LEGIDO-QUIGLEY C. Assessment of Chinese medicinal herb metabolite profiles by UPLC-MS-based methodology for the detection of aristolochic acids[J]. Journal of Separation Science,2007,30:1200-1206．
【11】MATUSZEWSKI B K,CONSTANZER M L,CHAVEZ-ENG C M. Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS[J]. Anal Chem,2003,75:3019-3030．
相关信息
