盐酸吖啶黄-脱氧核糖核酸反应体系的共振瑞利散射及其应用
Resonance Rayleigh Scattering of the Reaction System Between Acriflavine Hydrochloride and DNA and Its Analytical Application
摘 要
研究了盐酸吖啶黄与脱氧核糖核酸(DNA)之间的共振瑞利散射(RRS)增强作用,提出了共振瑞利散射技术测定核酸的方法.在pH 6.4的B-R缓冲溶液中,盐酸吖啶黄与脱氧核糖核酸结合使溶液共振瑞利散射强度增强,其最大散射峰位于505 nm处,而在330 nm波长处有一稍弱的散射峰.DNA质量浓度在0.04~0.80 mg·L-1范围内,与RRS强度呈线性关系,检出限(3S/N)为0.023 mg·L-1.应用于测定合成样品中DNA含量并测得回收率为98.0%~104.0%.初步探讨了反应机理,盐酸吖啶黄与DNA间的相互作用包含有静电引力、π-π堆积力.
脱氧核糖核酸
盐酸吖啶黄
Resonance rayleigh scattering
DNA
Acriflavine hydrochloride
Abstract
Based on the enhancing of resonance rayleigh scattering (RRS) due to the reaction between acriflavine hydrochloride and DNA,a method for the determination of DNA was proposed.Two RRS peaks,a strong peak at 505 nm and a weak peak at 330 nm in a B-R buffer medium of pH 6.4,were observed in the RRS spectrum.Linear relationship between values of RRS intensity and mass concentration of DNA was found in the range of 0.04-0.80 mg·L-1,and value of detection limit (3S/N) found was 0.023 mg·L-1.The proposed method was use in the determination of DNA in 3 simulated samples,giving recovery in the range of 98.0%-104.0%.Mechanism of the reaction was studied,and effects of electrostatic and π-π accumulation were found to be involved in the reaction.
中图分类号 O657.3
所属栏目 试验与研究
基金项目
收稿日期 2009/6/1
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备注徐红(1970-),女,贵州省凯里人,教授,主要研究方向为分子光谱分析.
引用该论文: XU Hong,LIU Shao-pu,LUO Hong-qun. Resonance Rayleigh Scattering of the Reaction System Between Acriflavine Hydrochloride and DNA and Its Analytical Application[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(1): 5～7
徐红,刘绍璞,罗红群. 盐酸吖啶黄-脱氧核糖核酸反应体系的共振瑞利散射及其应用[J]. 理化检验－化学分册, 2010, 46(1): 5～7
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【11】唐波,马骊,王洪鉴.新型主体化合物在生物活性分子分析中的应用新进展[J].分析化学,2002,30(4):482-485．
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