Chiral Separation of Enantiomers of 1-Methyl-3-Phenylpropylamine by High-performance Capillary Electrophoresis
摘 要
提出了高效毛细管电泳拆分手性药物中间体1-甲基-3-苯基丙胺的方法.研究了缓冲溶液pH值、手性选择剂质量浓度、柱温、电压等因素对分离度的影响.在缓冲溶液浓度15 mmol·L-1(NaH2PO4-Na2HPO4,pH 7.6)、运行电压15 kV、手性选择剂浓度为20 g·L-1、毛细管温度18 ℃条件下,在4 min内成功地分离了1-甲基-3-苯基丙胺对映体,其分离度为1.65.
Abstract
Based on a thorough study on the experimental conditiuons,including the acidity and buffer solution of the reaction medium,concentration of the selective chiral agent (hydroxypropyl-β-cyclodextrin,abbreviated as HP-β-CD),column temperature and separation voltage,a method for chiral separation of enantiomers of 1-methyl-3-phenylpropylamine by HP-capillary electrophoresis was proposed.Under the conditions of ① 15 mmol·L-1 phosphate buffer of pH 7.6,② using 20 g·L-1 HP-β-CD as selective chiral agent,③ temperature of the capillary column of 18 ℃ and ④ separation voltage of 15 kV,enantiomers of 1-methyl-3-phenylpropylamine were satisfactorily separated within 4 min,with value of Rs 1.65.
中图分类号 O657.8
所属栏目 工作简报
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收稿日期 2008/12/27
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备注李英杰(1961-),男,黑龙江人,教授,主要从事电泳、电色谱分析研究工作.
引用该论文: LI Ying-jie,SUN Peng,QIN Shi-li,BAI Gang. Chiral Separation of Enantiomers of 1-Methyl-3-Phenylpropylamine by High-performance Capillary Electrophoresis[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(2): 170~171
李英杰,孙鹏,秦世丽,柏刚. 高效毛细管电泳法分离1-甲基-3-苯基丙胺对映体[J]. 理化检验-化学分册, 2010, 46(2): 170~171
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参考文献
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【2】李洪霞,李伟,谷学新.毛细管电泳在手性分离中的应用进展[J].化学研究,2005,16(2):96-100.
【3】杨娟.柳胺节心定紧急降压作用的临床观察[J].辽宁医药学杂志,2003,17(1):42-43.
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【5】WREN C,STEPHEN A.Theoretical aspects of chiral separation in capillary electrophoresis,I initial evaluation of model[J].Chromatogr A,1992,603:235-241.
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