提出了毛细管电泳手性拆分β-受体阻滞剂美托洛尔、阿普洛尔、卡替洛尔的方法.考察手性拆分剂的种类及浓度、缓冲溶液的浓度及pH对手性拆分的影响.在95 mmol·L-1 Tris-H3PO4缓冲溶液中添加15 mmol·L-1羟丙基-β-环糊精(HP-β-CD)和5 g·L-1羧甲基-β-环糊精(CM-β-CD),美托洛尔对映体和阿普洛尔对映体分别在pH 3.2和pH 3.8获得基线分离,卡替洛尔对映体在同一分离条件下在pH 3.8获得较好分离,分离度达1.0.

A capillary electrophoresis was proposed for chiral separation of metoprolol,alprenolol and carteolol.The influences of types and concentration of chiralagent,pH and concentration of buffer solution used on the chiral separation were studied.In a buffer medium of 95 mmol·L-1 Tris-H3PO4 solution containing 15 mmol·L-1 hydroxypropyl-β-cyclodextrin (HP-β-CD) and 5 g·L-1 carboxy methyl-β-cyclodextrin (CM-β-CD),baseline separations of enantiomers of metoprolol and alprenolol were achieved at the acidities of pH 3.2 and pH 3.8 respectively.A good separation of enantiomers of carteolol was accomplished under the same condition at pH 3.8,attaining the degree of resolution of 1.0.