流动注射-化学发光法测定咖啡酸
FI-Chemiluminescence Determination of Caffeic Acid
摘 要
基于在酸性条件下,咖啡酸能增强高锰酸钾-罗丹明6G体系的化学发光强度,提出了测定咖啡酸的流动注射-化学发光法.在优化的试验条件下,咖啡酸的发光强度的增加值与其浓度在1.0×10-6~1.0×10-4mol·L-1范围内呈线性关系,检出限(3S/N)为2.4×10-8mol·L-1.方法用于咖啡酸片中咖啡酸含量的测定,回收率在98.0%~104.3%之间,相对标准偏差(n=11)为3.0%.
流动注射
高锰酸钾-罗丹明6G反应体系
咖啡酸
Chemiluminescence
Flow-injection
Reaction system of KMnO4-rohodamine 6G
Caffeic acid
Abstract
Based on the enhancement of chemiluminescence (CL) of the reaction system of KMnO4-rhodamine 6G by caffeic acid in an acidic medium,and by using the flow injection technique,a method of FI-CL determination of caffeic acid was proposed.Linear relationship between the magnitude of increase in CL intensity and mass concentration of caffeic acid was obtained in the range of 1.0×10-6-1.0×10-4mol·L-1 with detection limit (3S/N) of 2.4×10-8mol·L-1.The proposed method was used in the analysis of caffeic acid tablets,giving values recovery in the range of 98.0%-104.3% and RSD (n=11) of 3.0%.
中图分类号 O657.3
所属栏目 工作简报
基金项目 重庆市自然科学基金项目(CSTC-2007BB0367)
收稿日期 2009/1/30
修改稿日期
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备注廖伟(1984-),男,重庆长寿人,硕士研究生,研究方向为分子光谱分析.
引用该论文: LIAO Wei,LUO Hong-qun,LI Nian-bing. FI-Chemiluminescence Determination of Caffeic Acid[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(3): 286～288
廖伟,罗红群,李念兵. 流动注射-化学发光法测定咖啡酸[J]. 理化检验－化学分册, 2010, 46(3): 286～288
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【3】原忠,余江天,苏世文,等.用薄层扫描法测定中药狗脊和黑狗脊中原儿茶酸及咖啡酸的含量[J].沈阳药科大学学报,2000,17(5):338-340．
【4】吕元琦,邬春华,袁倬斌,等.毛细管电泳测定三精双黄连口服液中的黄芩甙元黄芩甙绿原酸和咖啡酸[J].分析测试学报,2004,23(4):98-100．
【5】ZHENG Jian-bin,LIU Xiao-xing,ZHOU Yuan-zhen,et al.Simultaneous determination of five phenolic compounds in dried flowers by LC using DAD combined electrochemical detection[J].Chromatographia,2007,65(11/12):707-712．
【6】TIAN S,NAKAMURA K,CUI T,et al.High-performance liquid chromatographic determination of phenolic compounds in rice[J].Journal of Chromatography A,2005,1063(1/2):121-128．
【7】LI Xin-pu,YU Jie,LUO Jin-yin,et al.Simultaneous determination of chlorogenic acid,caffeic acid,ferulic acid,protocatechuic acid and protocatechuic aldehyde in chinese herbal preparation by RP-HPLC[J].Chemical & Pharmaceutical Bulletin,2004,52(10):1251-1254．
【8】WANG Hua-fu,PROVAN G J,HELLIWELL K.Determination of rosmarinic acid and caffeic acid in aromatic herbs by HPLC[J].Food Chemistry,2004,87(2):307-311．
【9】SANTOS D P,BERGAMINI M F,FOGG A G,et al.Application of a glassy carbon electrode modified with poly(glutamic acid) in caffeic acid determination[J].Microchimica Acta,2005,151(1/2):127-134．
【10】REYES-SALAS E O,RANGEL-ORDONEZ L,MANZANILLA-CANO J A,et al.Voltammetric determination of caffeic acid in Arnica montana[J].Analytical Letters,2002,35(6):971-984．
【11】COSTIN J W,BARNETT N W,LEWIS S W,et al.Monitoring the total phenolic/antioxidant levels in wine using flow injection analysis with acidic potassium permanganate chemiluminescence detection[J].Analytic Chimica Acta,2003,499:47-56．
【12】曾昭琼.有机化学:上册[M].3版.北京:高等教育出版社,2001:292．
【13】曾昭琼.有机化学:上册[M].3版.北京:高等教育出版社,2001:61．
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