火焰原子吸收光谱法测定人血红细胞中锌、铁、镁
Direct Determination of Zinc,Iron,Magnesium in Human Red Blood Cells by FAAS
摘 要
人血红细胞样品经低渗Triton X-100溶液超声振荡溶血处理并稀释100倍后采用火焰原子吸收光谱法测定其中锌、铁和镁的含量.锌、铁和镁的检出限(3S/N)分别为2.44×10-3,2.85×10-2,1.56×10-3mg·L-1,相对标准偏差(n=7)分别为0.69%~1.03%,2.85%~3.84%,0.92%~1.22%.应用该法分析同一混合人血红细胞样品,测定结果与酸消化法测得的结果一致,3种元素的加标回收率为95.0%~110.0%之间.
人血红细胞
锌
铁
镁
FAAS
Human red blood cells
Zinc
Iron
Magnesium
Abstract
The sample of human red blood cell was treated to haemolyze with hypisotonic cold Triton X-100 solution by ultrasonic vibration.The the sample solution was diluted one hundred times,and the contents of Zn,Fe and Mg were directly determined by FAAS.The values of detection limit (3S/N) and RSD′s (n=7) for Zn,Fe and Mg were 2.44×10-3,2.85×10-2,1.56×10-3mg·L-1 and in the ranges of 0.69%-1.03%,2.85%-3.84%,0.92%-1.22%,respectively;The method was applied to the analysis of a same sample of mixed human red blood cell,and the results obtained were in consistency with those obtained by the acid digestion method.Values of recovery of the 3 elements were in the range of 95.0%-110.0%.
中图分类号 O657.31
所属栏目 试验与研究
基金项目 国家自然科学基金(30671770)
收稿日期 2009/5/19
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备注徐孝娜(1983-),女,陕西西安人,助理实验师,主要从事分析试验工作.
引用该论文: XU Xiao-na,LI Ya,GAO Shuang-bin,WANG Feng. Direct Determination of Zinc,Iron,Magnesium in Human Red Blood Cells by FAAS[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(4): 345～347
徐孝娜,李娅,高双斌,王枫. 火焰原子吸收光谱法测定人血红细胞中锌、铁、镁[J]. 理化检验－化学分册, 2010, 46(4): 345～347
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