反相高效液相色谱法测定油橄榄中山楂酸含量
RPHPLC Determination of Maslinic Acid in Spanish Oil Olive
摘 要
以枇杷叶为原料采用高效液相制备色谱技术制备了纯度达98.91%的山楂酸对照品,并用作制备标准曲线.油橄榄样品用乙醇-水(80+20)溶液90 ℃索氏回流提取24 h,在Kromasail C18色谱柱(250 mm×4.6 mm,5 μm)上分离,以甲醇-1%(体积分数)乙酸(88+12)混合溶液为流动相,流量1.0 mL·min-1,在215 nm波长处进行测定.此法用于油橄榄样品中山楂酸含量的检测,并在实样基础上加入山楂酸标准作方法的回收及精密度试验,测得平均回收率为98%,相对标准偏差(n=5)为1.21%.
山楂酸
西班牙产油橄榄
制备色谱
RP-HPLC
Maslinic acid
Spanish oil olive
HPLC preparation system
Abstract
The oil olive sample was extracted with C2H5OH-H2O (80+20 by vol.) in a Soxhlet′s extractor at 90 ℃ for 24 h.An aliquot of the extract was used for separation on Kromasail C18 column,and a mixture of methanol and acetic acid solution (1+99) mixed in the ratio of 88 to 12 by volume was used as mobile phase flowing through the column at a rate of 1.0 mL·min-1.UV-detection at the wavelength of 215 nm was used in the determination.Reference material of maslinic acid was prepared from leaves of Eriobotrya japonica by the HPLC preparation system,giving purity of the product attained to 98.91%,which was used as the exteranal standard.Recovery and precision of the method were tested by addition of standard maslinic acid (20.0 mg) separately to 5 samples of oil olive and analyzed by the method given,value of average recovery found was 98%,and value of RSD (n=5) found was 1.21%.
中图分类号 O658.1
所属栏目 工作简报
基金项目 合肥市人才资金项目(2007年);安徽省国际合作项目(09080703009)
收稿日期 2009/1/27
修改稿日期
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备注陈龙胜(1980-),男,安庆人,硕士,主要研究方向为天然产物提取、分离和生物转化.
引用该论文: CHEN Long-sheng,Lv Yang,XU Shu-wen,XIN Yang. RPHPLC Determination of Maslinic Acid in Spanish Oil Olive[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(4): 415～417
陈龙胜,吕杨,许舒雯,忻旸. 反相高效液相色谱法测定油橄榄中山楂酸含量[J]. 理化检验－化学分册, 2010, 46(4): 415～417
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参考文献
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【3】Ana Morquez Martin,Rocio De La Puerta Vozquez,Angeles Fernondez-arche,et al.Supressive effect of maslinic acid from pomace olive oil on oxidative stress and cytokine production in stimulated murine macrophages[J].Free Radic Res,2006,40(3):295-297．
【4】JUAN M E,PLANAS J M,RUIZ-GUTIERREZ V,et al.Antiproliferative and apoptosis-inducing effects of maslinic and oleanolic acids,two pentacyclic triterpenes from olives,on HT-29 colon cancer cells[J].Br J Nutr,2008,100(1):36-38．
【5】SCALON Cunha L C,ANDRADE E Silva M L,CARDOSO Furtado N A,et al.Antibacterial activity of triterpene acids and semi-synthetic derivatives against oral pathogens[J].Zeitschrift für Naturforschung C,2007,62(9/10):668-670．
【6】ULLAH F,HUSSAIN H,HUSSAIN J,et al.Tyrosinase inhibitory pentacyclic triterpenes and analgesic and spasmolytic activities of methanol extracts of Rhododendron collettianum[J].Phytother Res,2007,21(11):1076-1079．
【7】Fernando J Reyes-zurita,Eva E Rufino-palo Mares,Jose A Lupianez,et al.Maslinic acid,a natural triterpene from olea europaea L.,induces apoptosis in HT29 human colon-cancer cells via the mitochondrial apoptotic pathway[J].Cancer Letters,2009,273(1):44-46．
【8】孙宏斌,温小安,倪沛洲.科罗索酸和山楂酸的制备方法:中国,200410064929.7[P].2005-07-06．
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