试验表明:在pH 9.5氨水-氯化铵缓冲介质中,牛血红蛋白对过氧化氢与酸性络蓝K的氧化还原反应的催化作用因异丙肾上腺素的存在而被抑制,而且其抑制率与异丙肾上腺素的浓度在8.1×10-8~1.2×10-5mol·L-1范围内呈线性关系,其检出限(3S/N)为2.2×10-9mol·L-1.据此,提出了一种简单而灵敏的测定异丙肾上腺素的酶催化光度法.此方法已用于盐酸异丙肾上腺素注射液中异丙肾上腺素含量的测定,并以此样品为基体用标准加入法作回收试验,测得回收率在92.8%~105.4%之间,测定值的相对标准偏差(n=5)在2.6%~5.8%之间.

It was found that in an ammoniacal buffer medium of pH 9.5,the catalytic effect of bovine hemoglobin on the redox reaction between hydrogen peroxide and acid chrome blue K was inhibited by the presence of isoprenaline,and that linear relationship was kept between values of rate of inhibition and concentration of isoprenaline in the range of 8.1×10-8-1.2×10-5mol·L-1 with its detection limit (3S/N) of 2.2×10-9mol·L-1.On these basis,a sensitive and simple photometric method for determination of isoprenaline was proposed.The proposed method was used for the determination of isoprenaline in its injections and recovery of the method was tested by standard addition method using these samples as matrixes.Values of recovery found were ranged from 92.6% to 105.4% with values of RSD′s (n=5) in the range of 2.6%-5.8%.