加压毛细管电色谱法分离美西律对映体
Resolution of Enantiomer of Mexiletine by Pressurized Capillary Electrochromatography
摘 要
采用加压毛细管电色谱法,以羟丙基-β-环糊精作为手性选择剂,对美西律对映体进行手性分离.在优化的试验条件下,16 g·L-1羟丙基-β-环糊精为手性选择剂,pH 2.5的乙腈与10 mmol·L-1磷酸盐缓冲溶液以体积比1比1混合溶液为流动相,运行电压10 kV,毛细管柱温20 ℃,美西律对映体在4 min内成功分离,分离度为1.51.
盐酸美西律
对映体拆分
羟丙基-β-环糊精
Pressurized capillary electrochromatography
Mexiletine hydrochloride
Resolution of enantiomers
Hydroxypropyl -β-cyclodextrin
Abstract
A method of pressurized capillary electrochromatography was proposed for the resolution of enantiomers of mexiletine using hydroxypropyl-β-cyclodextrin (16 g·L-1) as selective chiral agent.A mixed solution of acetonitrile and 10 mmol·L-1 phosphate buffer solution(1+1 by vol.) of pH 2.5 was used as mobile phase.The separation voltage of 10 kV and temperature of capillary column of 20 ℃ were chosen.Under the optimized conditions,the enantiomer of mexiletine was successfully separated within 4 min with resolution factor of 1.51.
中图分类号 O652.63
所属栏目 工作简报
基金项目
收稿日期 2009/1/26
修改稿日期
网络出版日期
作者单位点击查看
备注李英杰(1961-),男,黑龙江人,教授,主要从事电泳、电色谱分析研究工作.
引用该论文: LI Ying-jie,SUN Peng,GAO Yu-lin,GAO Li-di. Resolution of Enantiomer of Mexiletine by Pressurized Capillary Electrochromatography[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(4): 441～442
李英杰,孙鹏,高玉玲,高立娣. 加压毛细管电色谱法分离美西律对映体[J]. 理化检验－化学分册, 2010, 46(4): 441～442
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】LANCHOTE V I,BONATO P S.High-perfomance liquid chromatographic detemination of mexiletine enantiomers in plasma using direct and indirect enantioselective eparations[J].J Chrarnatogr B,1996,685:281-289．
【2】MEHVAR R,BROCKS D R,VAKILY M.Impact of stereoselectivity on the phamracokinetics and phamracodynamics of antiarrhythmic drugs[J].Clin Phannacokinet,2002,41(8):533-558．
【3】康经武,阮宗琴,欧庆瑜,等.盐酸美西律对映体的毛细管电泳分离[J].分析化学,1997,25(3):290-293．
【4】曹枫,张维冰,阎超,等.压力对加压电色谱分离选择性影响的研究[J].分析化学,2004,32(2):143-147．
【5】STRICKMANN B C,GOTTFRIED B,CLAUDIA D,et al.Separation and identification of etodolac and its urinary phase Ⅰ metabolites using capillary electrochromatography and on-line capillary electrochromatography-electrospray ionisation mass spectrometry coupling[J].J Chromatogr A,2000,887(1/2):393-407．
【6】RU Qin-hua,LUO Guo-an,FU Yu-rong.Effect of various modes of pressurization on the separation in capillary electrochromatography[J].J Chromatogr A,2001,924(1/2):331-336．
【7】GERALD G,MARTIN G S.Chiral separation by capillary electromigration techniques[J].J Chromatogr A,2008,1204(2):140-156．
【8】JOSEPH J P,MARA T M,DIPTI S.Capillary liquid chromatography and capillary electrochromatography using silica hydride stationary phases[J].J Chromatogr A,2008,1191(1/2):136-140．
【9】王志,孙亦梁,孙曾培.高效毛细管电泳分离手性物质[J].分析测试学报,1996,15(2):85-92．
【2】MEHVAR R,BROCKS D R,VAKILY M.Impact of stereoselectivity on the phamracokinetics and phamracodynamics of antiarrhythmic drugs[J].Clin Phannacokinet,2002,41(8):533-558．
【3】康经武,阮宗琴,欧庆瑜,等.盐酸美西律对映体的毛细管电泳分离[J].分析化学,1997,25(3):290-293．
【4】曹枫,张维冰,阎超,等.压力对加压电色谱分离选择性影响的研究[J].分析化学,2004,32(2):143-147．
【5】STRICKMANN B C,GOTTFRIED B,CLAUDIA D,et al.Separation and identification of etodolac and its urinary phase Ⅰ metabolites using capillary electrochromatography and on-line capillary electrochromatography-electrospray ionisation mass spectrometry coupling[J].J Chromatogr A,2000,887(1/2):393-407．
【6】RU Qin-hua,LUO Guo-an,FU Yu-rong.Effect of various modes of pressurization on the separation in capillary electrochromatography[J].J Chromatogr A,2001,924(1/2):331-336．
【7】GERALD G,MARTIN G S.Chiral separation by capillary electromigration techniques[J].J Chromatogr A,2008,1204(2):140-156．
【8】JOSEPH J P,MARA T M,DIPTI S.Capillary liquid chromatography and capillary electrochromatography using silica hydride stationary phases[J].J Chromatogr A,2008,1191(1/2):136-140．
【9】王志,孙亦梁,孙曾培.高效毛细管电泳分离手性物质[J].分析测试学报,1996,15(2):85-92．
相关信息
