荧光免疫法测定鸡肉中己烯雌酚残留
Determination of Residual Diethylstilbestrol in Chicken by Fluoroimmunoassay
摘 要
用制备的抗己烯雌酚抗体包被酶标板,以双抗夹心免疫响应模式为基础,异硫氰酸荧光素为荧光探针,提出了抗己烯雌酚抗体包被模式荧光免疫法测定鸡肉中己烯雌酚残留量.己烯雌酚的质量浓度的对数值(lgρ)与其荧光强度在0.1~1.0×103μg·L-1范围内呈线性关系,检出限(3S/N)为50 ng·L-1.此法用于鸡肉样品的分析,回收率在85.5%~113.8%之间.
己烯雌酚
荧光探针
Fluoroimmunoassay
Diethylstilbestrol
Fluorescence probe
Abstract
The prepared anti-diethylstilbestrol (DES) antibody was used to coat ELISA plates,and isothiocyanic acid fluorescein was used as the fluorescence probe.Based on double-antibody sandwich immune response model,a method for the determination of DES in chicken by fluoroimmunoassay was described.Linear relationship between values of fluorescence intensity and logarithm of mass concentration (lgρ) of DES was obtained in the range of 0.1-1.0×103μg·L-1 with detection limit (3S/N) of 50 ng·L-1.The proposed method was applied to the analysis of samples of chicken,giving values of recovery in the range of 85.5%-113.8%.
中图分类号 O657.3
所属栏目 试验与研究
基金项目 国家自然科学基金(20877037);山东省自然科学基金(2005ZX12);山东省泰山学者建设工程基金的资助
收稿日期 2009/3/20
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备注孙梅梅(1981-),女,山东淄博人,硕士研究生,主要从事光分析化学研究.
引用该论文: SUN Mei-mei,ZHAO Yan-wei,LIANG Yan,QIAN Jian-rui,LI Li-hua,WANG Shu-hao. Determination of Residual Diethylstilbestrol in Chicken by Fluoroimmunoassay[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(5): 465～467
孙梅梅,赵艳伟,梁艳,钱建瑞,李立华,王术皓. 荧光免疫法测定鸡肉中己烯雌酚残留[J]. 理化检验－化学分册, 2010, 46(5): 465～467
被引情况:
【1】杜凌云,朱琳,王术皓, "荧光光度法测定己烯雌酚",理化检验－化学分册 49, 985-987(2013)
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参考文献
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【2】WOJTON B.Animal diseases spread by food[J].Pizegl Epidemiol,2001,55(2):1-4．
【3】GINSTI R M,IVAMOTO K,HATCH E E.Diethylstilbestrol revisited:a review of the long term health effects[J].Ann Interm Med,1995,122:778-788．
【4】李铠年,姜荃.国外对食品药物残留控制的发展趋势[J].世界农业,2003,5:44-46．
【5】沈美芳,杨利国,丁炜东.动物性食品中己烯雌酚残余量检测方法评定[J].动物科学与动物医学,2004,21(5):24-26．
【6】张彩虹.高效液相色谱法测定己烯雌酚的实验研究[J].中国热带医学,2006,6(2):328-329．
【7】吴银良,刘素英,单吉浩,等.气相色谱-质谱法分析鸡肝中己烯雌酚残余量[J].中国兽药杂志,2005,39(11):8-10．
【8】Sara Rodriguze-Mozaz,LPEZ DE ALDA M J,et al.Monitoring of estrogens,pesticides and bisphenol A in natural waters and drinking water treatment plants by solid-phase extraction-liquid chromatography-mass spectrometry[J].Journal of Chromatography A,2004,1045:85-92．
【9】邢丽,李伟.薄层色谱法鉴别雌氯乳膏中氯霉素和己烯雌酚[J].华西药学杂志,2006,21(2):212．
【10】MOSLEMI S,SILBERZAHN P,SRALINI G E.Measurements of sterorid and-agonist concentrations in infant milks[J].Lait,1996,76(6):537-550．
【11】胡锟,杨先乐,张菊.酶联免疫分析法检测中华鳖肌肉中己烯雌酚[J].上海水产大学报,2002,11(3):199-202．
【12】LIAO Su-lan,WU Xiao-ping,XIE Zhen-hong.Determination of some estrogens by flow injection analysis with acidic potassium permanganate-formaldehyde chemiluminescence detection[J].Anal Chim Acta,2005,537:189-195．
【13】王术皓,庄惠生,姚型军,等.流动注射阻抑化学发光法测定己烯雌酚[J].分析试验室,2006,25(8):10-12．
【14】朱将伟,柴春彦,刘国艳,等.以CTAB作电子介体伏安法检测饲料中的己烯雌酚[J].粮食与饲料工业,2006,8:43-44．
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