提出了反相高效液相色谱法同时测定功能饮料中6种B族维生素,维生素B1、维生素B2、维生素B6、烟酸、烟酰胺和泛酸的方法.对色谱分离条件做了试验并予以优化.以Kromasil C18色谱柱(200 mm×4.6 mm,5 μm)为分离柱,用甲醇(A)和0.3%(体积分数)三乙胺溶液(B)(磷酸调节pH至3.5)以不同比例混合进行梯度淋洗,选择在200 nm波长处测定泛酸,在270 nm波长处测定其他5种B族维生素.6种B族维生素的质量浓度在0.5~100 mg·L-1范围内与其峰面积呈线性关系.标准加入回收率在97.8%~106.2%之间,相对标准偏差(n=5)在0.5%~2.3%之间.

RP-HPLC was applied to the simultaneous determinations of six B-group vitamins including vitamin B1,vitamin B2,vitamin B6,niacin,nicotinamide and pantothenic acid in functional drinks.The conditions of separation by liquid chromatography were tested and optimized.The Kromasil C18 column (200 mm×4.6 mm,5 μm) was used as stationary phase,and mixtures of methanol (A) and triethylamine solution (φ=0.3%,adjusted to pH 3.5 with H3PO4) (B) in different ratios were used as mobile phase in the gradient elution.UV-detections were used at the wavelength of 200 nm for determination of pantothenic acid and at 270 nm for the other 5 vitamin B′s.Linear relationships between the values of peak area and mass concentration of the six B-group vitamins were obtained in the range of 0.5~100 mg·L-1.The values of recovery found by standard addition method were in the range of 97.8%-106.2%,and values of RSD′s (n=5) were in the range of 0.5%-2.3%.