提出了用高效液相色谱法同时测定槐枝中芦丁和槲皮素含量的方法.采用ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5 μm)为固定相,以甲醇-0.01 mol·L-1乙酸铵(47+53)混合溶液为流动相,在360 nm波长处用二极管阵列检测器检测.芦丁和槲皮素的质量分别在0.015 4~0.154 0,0.010 8~0.108 0 mg·L-1之间与其相应的峰面积呈线性关系,检出限分别为(3S/N)分别为0.06,0.008 μg·g-1.应用此法测定槐枝样品中芦丁和槲皮素的含量,加标回收率分别为97.4%~98.8%,97.4%~98.2%;相对标准偏差(n=6)分别为1.6%,1.8%.

HPLC was applied to the simultaneous determination of rutin and quercetin in branches of Sophora aponica L.ZORBAX Eclipse XDB-C18 column (4.6 mm×250 mm,5 μm) was used as stationary phase,and a mixture of methanol and 0.01 mol·L-1 ammonium acetate solution (47+53) was used as mobile phase in the HPLC analysis with DAD detection at the wavelength of 360 nm.Linear relationships between values of peak area and mass of rutin and quercetin were kept in ranges of 0.015 4-0.154 0 mg·L-1 and 0.010 8-0.108 0 mg·L-1 with detection limits (3S/N) of 0.06,0.008 μg·g-1 respectively.Nine samples were analyzed for contents of rutin and quercetin by the proposed method,values of recovery found by standard addition method were in ranges of 97.4%-98.8% and 97.4%-98.2%,and values of RSD′s (n=6) found were 1.6% and 1.8%,respectively.