Flow Injection Biamperometric Determination of Silymarin
摘 要
使用一支经恒电位预阳极化处理的双铂电极,在外加电位差为0.2 V时,通过偶合水飞蓟素在一支电极上的氧化和氧化铂在另一支电极上的还原两个不可逆电极过程,在此基础上并结合流动注射技术提出了流动注射双安培法直接测定水飞蓟素的方法.在pH 5.02 B-R缓冲溶液中,水飞蓟素氧化峰电流与其浓度在4.0×10-5~8.0×10-4mol·L-1范围内呈线性关系,检出限(3S/N)为2.0×10-5mol·L-1.连续20次测定1.0×10-4mol·L-1水飞蓟素标准溶液,所得相对标准偏差为3.3%.方法用于益肝灵片中水飞蓟素含量的测定,回收率为99.0%.
Abstract
Under the conditions of applying the external potentials onto the pretreated binary Pt-electrodes having a potential difference ΔE of 0.2 V,and coupling the 2 irreversible electrode processes of the oxidation of silymarin at one of the Pt electrode and the reduction at the other Pt-electrode which is coated with PtO through the anodization treatment,linear relationship between values of peak current Ip and concentration of silymarin was obtained in the range of 4.0×10-5-8.0×10-4mol·L-1.Based on these facts and by applying the flow-injection technique,a method of direct FI-biamperometric determination of silymarin was proposed.The reaction was carried out in a B-R buffer medium of pH 5.02,and the detection limit (3S/N) of the method found was 2.0×10-5mol·L-1.Precision was tested at the concentration level of 1.0×10-4mol·L-1 of silymarin for 20 determinations,giving the value of RSD 3.3%.The proposed method was applied to the analysis of a silymarin-containing drug,giving value of recovery of silymarin 99.0%.
中图分类号 O657.1
所属栏目 工作简报
基金项目 陕西省科技厅项目(2005B01);陕西省教育厅基金项目(06JK164);咸阳师范学院专项科研基金资助重点项目(05XSYK105)
收稿日期 2009/4/30
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备注刘彬(1958-),男,陕西三原人,教授,博士,研究方向为电分析化学.
引用该论文: LIU Bin,YANG Bo-lun,SUN Jia-juan. Flow Injection Biamperometric Determination of Silymarin[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(7): 772~774
刘彬,杨伯伦,孙家娟. 流动注射双安培法测定水飞蓟素[J]. 理化检验-化学分册, 2010, 46(7): 772~774
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【3】李风前.水飞蓟素固体分散体中总黄酮的测定[J].中草药,2002,33(2):31-33.
【4】吴知行.水飞蓟宾及其水溶性衍生物和制剂含量测定方法的研究[J].南京药学院学报,1981,12(2):28-36.
【5】ZHAO Chuan,ZHANG Jun-cai,SONG Jun-feng.Determination of L-cysteine in amino acid mixtures and human urine by flow-injection analysis with a biamperometric detector[J].Anal Biochem,2001,297(2):170-172.
【6】孙家娟,尚永辉,刘彬,等.流动注射双安培法测定辛弗林[J].分析科学学报,2007,23(2):201-204.
【7】张君才,赵川,宋俊峰.流动注射双安培法测异烟肼[J].药学学报,2001,36(9):679-681.
【8】张君才,刘静,白育伟,等.流动注射双安培法测定维生素P[J].理化检验-化学分册,2005,41(6):393-395.
【9】宋俊峰,赵川,过玮,等.抗坏血酸的双安培流动注射分析[J].分析化学,2000,28(1):38-40.
【10】赵川,张君才,宋俊峰.流动注射双安培法直接测定茶叶中单宁[J].分析化学,2002,30(2):210-213.
【11】孙家娟,尚永辉,刘彬,等.流动注射双安培法测定葛根素[J].分析测试学报,2007,26(2):225-227.
【12】陈家全,宋俊峰,张雪红.流动注射不可逆双安培法测定二苯胺磺酸盐[J].理化检验-化学分册,2004,40(10):567-570.
【13】宋俊峰,赵川,亢晓峰,等.两个不可逆电对体系的双安培流动注射分析[J].高等学校化学学报,2000,21(4):535-537.
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