提出了快速测定油脂食品、焙烤食品和方便米面食品中抗坏血酸棕榈酸酯的高效液相色谱法.以柠檬酸和异抗坏血酸为稳定剂,甲醇为样品提取剂,以甲醇-乙腈(1+1)及磷酸(1+99)溶液为流动相,经反相C18色谱柱梯度洗脱,在243 nm波长处,用光电二极管阵列检测器进行测定.抗坏血酸棕榈酸酯的质量浓度在0.50~100.0 mg·L-1范围内与其峰面积呈线性关系,测定下限(10S/N)为0.005 g·kg-1.加标回收率在85%~104%之间,相对标准偏差(n=6)在1.1%~3.9%之间.

High performance liquid chromatography was applied to the determination of ascorbyl palmitate in fatty food,baked food,and ready-to-eat food. The sample was extracted with methanol using citric acid and iso-ascorbic acid as stabilizing agent. The extract obtained was separated on the reversed phase C18 column with mixtures of phosphoric acid (1+99) and mixed solvent of acetonitrile and methanol (1+1) in different ratios as mobile phase in gradient elution. UV-detection at 243 nm with photodiode array detector (PAD) was used in the determination. Linear relationship between values of peak area and mass concentration of ascorbyl palmitate was kept in the range of 0.50-100.0 mg·L-1. Lower limit of determination (10S/N) of the method was found to be 0.005 g·kg-1. Test for recovery were made by standard addition method,giving values of recovery in the range of 85%-104% and RSD′s (n=6) in the range 1.1%-3.9%.