Comparative Study on Methods for Determination of Glutathione in Fermentation Liquor
摘 要
用离心法从谷胱甘肽(GSH)产生菌发酵液中分离收集菌体.分别用冻融提取法,乙醇提法和水提法从菌体中提取GSH,所得提取液中GSH含量分别用碘量法、DTNB衍生-光度法和高效液相色谱法(HPLC)测定.对3种方法所测得结果作了比较,结果表明:HPLC法测定,由于可避免共存的其他抗氧化剂和含有巯基化合物的干扰,可达到最好的准确性和选择性.因此,其测定结果与其他两种方法相比,最准确可靠.DTNB衍生-光度法的测定结果稍高但接近于HPLC的结果,其优点是比较简单,测试成本较低,适合于大规模生产的控制分析.
Abstract
Bacteriotome was collected from the bacteria-producing fermentation liquor of glutathione (GSH) by centrifugation,and the GSH was extracted from the bacteriotome by the method of freeze-thaw extraction,by the method of alcohol extraction and by the method of water extraction. Content of GSH in the extract,obtained by either of the 3 above mentioned methods,was then determined by the iodometric titration,the DTNB photometric method,and the HPLC method separately. A comparative study on the results found by the 3 methods was made,and it was shown that best accuracy and selectivety was achieved by the HPLC method,as the interferences from other co-existing anti-oxidants and sulfhydryl-containing compounds were effectively eliminated in this method,and most accurate results were obtained as compared with the other 2 methods. Results obtained by the DTNB photometric method were a little bit higher but quite closer to the results of the HPLC method,and the DTNB photometric method was found to be feasible for routine analysis in large scale production.
中图分类号 O652.63
所属栏目 工作简报
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收稿日期 2009/5/22
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备注陈龙(1980-),男,湖北武汉人,助理工程师,研究方向为多肽提取与纯化.
引用该论文: CHEN Long,YUAN Hong-Li,CHEN Dong-Liang,YU Lan. Comparative Study on Methods for Determination of Glutathione in Fermentation Liquor[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(8): 876~878
陈龙,袁宏丽,陈栋梁,于兰. 发酵液中谷胱甘肽含量测定方法的比较[J]. 理化检验-化学分册, 2010, 46(8): 876~878
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参考文献
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