基于与氯冉酸的荷移反应-分光光度法测定甲氧氯普胺含量
Spectrophotometric Determination of Metoclopramide Based on Its Charge Transfer Reaction with Chloranilic Acid
摘 要
试验表明:甲氧氯普胺与氯冉酸在乙醇介质中,室温条件下即可形成稳定的1∶1型荷移络合物,该络合物的最大吸收波长为532 nm,表观摩尔吸光率为1.21×103L·mol-1·cm-1.甲氧氯普胺的质量浓度在10.0~300.0 mg·L-1范围内服从比耳定律.用甲氧氯普胺标准溶液平行测定6次,其测定值的相对标准偏差为0.58%.按此方法分析了2个不同批号的甲氧氯普胺药物,所得结果与药典法测定结果相符.以此样品作基体用标准加入法作回收试验,求得回收率在97.8%~101.5%之间.
荷移反应
甲氧氯普胺
氯冉酸
Spectrophotometry
Charge transfer reaction
Metoclopramide
Chloranilic acid
Abstract
It was found that in an ethanol medium,a charge transfer complex with mole ratio of 1 to 1 was formed by the reaction between metoclopramide and chloranilic acid,showing its absorption maximum at the wavelength of 532 nm. Apparent molar absorptivity of 1.21×103L·mol-1·cm-1 was found,and Lambert. Beer′s law was kept in the range of 10.0-300.0 mg·L-1 for metoclopramide. Precision of the method was tested with standard solution of metoclopromide for 6 determinations,giving value of RSD 0.58%. Two samples of metoclopromide from different batches were analyzed by the proposed method giving results in consistency with results found by the pharmacopeia method and recovery was tested by standard addition method with these samples as matrixes,the values of average recovery found were in the range of 97.8%-101.5%.
中图分类号 O657.31
所属栏目 工作简报
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收稿日期 2009/4/25
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备注于丽丽(1979-),女,辽宁朝阳人,讲师,硕士,主要研究方向药物分析及天然药物化学.
引用该论文: YU Li-li,LI Hua-kan,LIU Jia-chuan,WANG Yu-hua. Spectrophotometric Determination of Metoclopramide Based on Its Charge Transfer Reaction with Chloranilic Acid[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(8): 906～907
于丽丽,李华侃,刘佳川,王玉华. 基于与氯冉酸的荷移反应-分光光度法测定甲氧氯普胺含量[J]. 理化检验－化学分册, 2010, 46(8): 906～907
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参考文献
【1】范顺利,张磊,吴志皓,等.流动注射化学发光法测定甲氧氯普胺[J].分析试验室,2003,22(2):48-50．
【2】BUNA M,AARON J J,PROGNON P,et al. Effects of pH and solvent on the fluorescence properties of biomedically important benzamides. Application to determination in drugs and in human urine[J]. Analyst,1996,121(11):1551-1556．
【3】刘惠军,吴丽红,张继春.高效液相色谱法测定甲氧氯普胺及其片剂的含量和有关物质[J].药物分析杂志,2008,28(4):559-561．
【4】BADAWY S S,SHOUKRY A F,ISSA Y M. Ion-selective electrode for the determination of metoclopramide[J]. Analyst,1986,111(12):1363-1365．
【5】国家药典委员会.中华人民共和国药典(二部)[M].北京:化学工业出版社,2005:119．
【6】周旭光,冯建章,童沈阳.荷移反应及其在分析化学中的应用Ⅱ.氟哌酸测定方法研究[J].分析化学,1993,21(2):184-186．
【7】李华侃,赵桂芝,赵延清,等.克霉唑与氯冉酸的荷移反应及其测定[J].分析化学,2002,30(3):334-336．
【2】BUNA M,AARON J J,PROGNON P,et al. Effects of pH and solvent on the fluorescence properties of biomedically important benzamides. Application to determination in drugs and in human urine[J]. Analyst,1996,121(11):1551-1556．
【3】刘惠军,吴丽红,张继春.高效液相色谱法测定甲氧氯普胺及其片剂的含量和有关物质[J].药物分析杂志,2008,28(4):559-561．
【4】BADAWY S S,SHOUKRY A F,ISSA Y M. Ion-selective electrode for the determination of metoclopramide[J]. Analyst,1986,111(12):1363-1365．
【5】国家药典委员会.中华人民共和国药典(二部)[M].北京:化学工业出版社,2005:119．
【6】周旭光,冯建章,童沈阳.荷移反应及其在分析化学中的应用Ⅱ.氟哌酸测定方法研究[J].分析化学,1993,21(2):184-186．
【7】李华侃,赵桂芝,赵延清,等.克霉唑与氯冉酸的荷移反应及其测定[J].分析化学,2002,30(3):334-336．
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