反相高效液相色谱法测定18F-FECNT的前体化合物nor-CClT
RPHPLC Determination of nor-CClT the Precursor in Preparation of the Labelled Compound 18F-FECNT
摘 要
提出了用反相高效液相色谱法测定18F-FECNT的标记前体化合物nor-CClT[2β-甲酯基-3β-(4氯苯基)去甲基托烷]的含量.采用Lichrospher C18色谱柱作为固定相,用甲醇、水及三乙胺以体积比为65比35比0.21混合的溶液作为流动相进行分离.测定中采用紫外检测波长为230 nm,在此条件下,nor-CClT达到了与共存杂质间的良好分离,所测得峰面积值与nor-CClT的质量浓度在0.100~1.200 g·L-1范围内呈线性关系.取0.400,0.600,1.000 g·L-1 nor-CClT标准溶液做精密度试验,测得日内相对标准偏差(n=5)依次为0.53%,0.46%,0.32%.
nor-CClT
18F-FECNT
HPLC
nor-CClT
18F-FECNT
Abstract
A method of RP-HPLC was proposed for the determination of nor-CClT [or in its whole chemical name,2β-carbomethoxy-3β-(4-chlorophenyl) nortropane] which is the precursor in preparation of the labelled compound,18F-FECNT,or in its whole chemical name Fluoro [18F]-N-2-fluoroethyl-2β-carbomethoxy-3β-(4-chlorophenyl) nortropane. The Lichrospher C18 column was used as stationary phase,a mixture of methanol,water and triethylamine mixed in the ratio of 65 to 35 to 0.21 (by vol.) was used as mobile phase and UV-detection at the wavelength of 230 nm was adopted in the determination. Under the optimum condition,clear separation of nor-CClT from the co-existing impurities was attained. Linear relationship between values of peak area and mass concentration of nor-CClT was obtained in the range of 0.100 to 1.200 g·L-1. Values of intraday RSD′s (n=5) found by testing at 3 concentration levels of 0.400,0.600,1.000 g·L-1 were 0.53%,0.46% and 0.32% respectively.
中图分类号 O657.7
所属栏目 工作简报
基金项目 国家自然科学基金(30570518;30970844);江苏省高新技术研究计划(BG2007603);江苏省卫生厅科研基金(H200401)
收稿日期 2009/4/26
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备注唐婕(1983-),女,无锡人,主要研究方向为放射性药物分析.
引用该论文: TANG Jie,CHEN Zheng-ping,LI Xiao-min,WANG Song-pei,LIU Chun-yi,QIN Xiao-feng,ZHOU Xing-qin. RPHPLC Determination of nor-CClT the Precursor in Preparation of the Labelled Compound 18F-FECNT[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(8): 914～916
唐婕,陈正平,李晓敏,王颂佩,刘春仪,钦晓峰,周杏琴. 反相高效液相色谱法测定18F-FECNT的前体化合物nor-CClT[J]. 理化检验－化学分册, 2010, 46(8): 914～916
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