采用火焰原子吸收光谱法对复方丹参片水煎液及人工胃酸提取液中具有生物活性的微量元素铁、铜、锰和锌的形态分布进行了研究.用Oasis TMHLB固相提取小柱分离药物的两种提取液中所述4种元素的无机态和有机态；用D401螯合树脂分离4种元素的稳定态和不稳定态；用Amberlite XAD-7大孔吸附树脂分离得到与丹酚酸的有机结合态.结果表明:锌、铜的蛋白质结合态分布较高,多糖结合态中以铜元素为主,丹酚酸有机结合态中铁元素的含量最高.

Various species (including soluble,inorganic,organic,stable,unstable,protein-binding,polysaccharide-binding and organic-binding states) of the bioactive trace elements (i.e.,Fe,Cu,Mn and Zn) in decoction and in solution extracted with simulated gastric acid from the compound tablets of roots of Salvia miltiorrhiza were separated and determined by FAAS. Species of inorganic and organic state were separated on the OasisTM HLB SPE column,and the stable and unstable species were separated on the D401 chelating resin column. The organic binding species i.e. bound mainly with salvianolic acid in the filtrate of the aqueous decoction of the drug was separated by the Amberlite XAD-7 resin column. It was found that higher contents of Zn and Cu were found in the protein binding species;Cu was the main element found in the polysaccharide-binding species;and among the 4 elements in the eluate from Amberlite XAD-7 column,content of Fe was the highest.