HPLC Determination of 8 Synthetic Colorants in Canned Yellow Peach
摘 要
应用高效液相色谱法测定了黄桃罐头中柠檬黄、苋菜红、靛蓝、胭脂红、日落黄、诱惑红、亮蓝和赤藓红等8种人工合成着色剂的含量.样品用乙醇-氨水-水溶液提取后,经Sep-Pak Plus QMA固相萃取柱净化处理,以甲醇和乙酸铵溶液为流动相梯度洗脱,经Inertsil ODS-3V(4.6 mm×250 mm,5 μm)色谱柱分离,用二极管阵列检测器检测.8种着色剂的质量浓度在0.1~20.0 mg·L-1范围内与其峰面积呈线性关系,测定下限(10S/N)在0.20~0.50 mg·kg-1之间.方法对8种着色剂的平均回收率分别为柠檬黄94.3%~96.2%、苋菜红93.3%~99.6%、靛蓝77.9%~84.7%、胭脂红95.0%~101.0%、日落黄94.6%~103.0%、诱惑红94.1%~104.0%、亮蓝96.4%~104.0%、赤藓红85.9%~89.7%,相对标准偏差(n=6)均小于9.0%.
Abstract
HPLC was applied to the determination of 8 synthetic colorants,including tartrazine,amaranth,indigotin,ponceau 4R,sunset yellow,allura red,brilliant blue and erythrosine,in canned yellow peach.The sample was extracted with aqueous ethanol-ammonia solution and the extract was purified on Sep-Pak Plus QMA SPE column.The eluate from the column was separated by gradient elution with methanol and ammonium acetate mixed solutions of different ratio on Inertsil ODS-3V (4.6 mm×250 mm,5 μm) column,and determined with diode array detector.Linear relationships between values of peak areas and mass concentrations of the 8 synthetic colorants were obtained in the range of 0.1-20.0 mg·L-1,with their lower limits of determination (10S/N) in the range of 0.25 to 0.5 mg·kg-1.The values of average recovery of 8 synthetic colorants found were in the range of 94.3%-96.2% for tartrazine,93.3%-99.6% for amaranth,77.9%-84.7% for indigotine,95.0%-101.0% for ponceau 4R,94.6%-103.0% for sunset yellow,94.1%-104.0% for allura red,96.4%-104.0% for brilliant blue,85.9%-89.7% for erythrosine.The values of RSD′s (n=6) found were less than 9.0%.
中图分类号 O652.63
所属栏目 工作简报
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收稿日期 2009/8/5
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备注王冬妍(1977-),女,黑龙江人,工程师,硕士,主要从事食品检验及研究工作.
引用该论文: WANG Dong-yan,YANG Xiao-li,LI Xing-quan,YANG Shu-ning. HPLC Determination of 8 Synthetic Colorants in Canned Yellow Peach[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(9): 1034~1036
王冬妍,杨晓丽,李兴权,杨书宁. 高效液相色谱法测定黄桃罐头中8种合成着色剂[J]. 理化检验-化学分册, 2010, 46(9): 1034~1036
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参考文献
【1】GB/T 5009.35-2003食品中合成着色剂的测定方法[S].
【2】郑毅,张宜友,刘淳.食品中合成色素检验技术[J].中国卫生检验杂志,2000,10(6):663-667.
【3】刘奋,林奕芝,戴京晶,等.高效液相色谱多波长测定人工合成着色剂[J].现代预防医学,2001,28(1):81-82.
【2】郑毅,张宜友,刘淳.食品中合成色素检验技术[J].中国卫生检验杂志,2000,10(6):663-667.
【3】刘奋,林奕芝,戴京晶,等.高效液相色谱多波长测定人工合成着色剂[J].现代预防医学,2001,28(1):81-82.
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