荧光光谱法研究2,4,6-三氯苯酚与胰蛋白酶的相互作用
Study on the Interaction of 2,4,6-Trichlorophenol and Trypsin by Fluorescence Spectrometry
摘 要
运用荧光光谱法研究了2,4,6-三氯苯酚与胰蛋白酶的相互作用.结果表明:2,4,6-三氯苯酚通过静电和疏水作用力与胰蛋白酶形成基态复合物导致胰蛋白酶内源荧光猝灭,猝灭机理主要为静态猝灭.计算了该反应的表观结合常数K、结合位点数n及结合反应的热力学参数,并用同步荧光和三维荧光技术考察了2,4,6-三氯苯酚对胰蛋白酶构象的影响,酪氨酸和色氨酸残基所处微环境的疏水性增加.
结合反应
2,4,6-三氯苯酚
胰蛋白酶
Fluorescence spectrosmetry
Binding reaction
2,4,6-Trichlorophenol
Trypsin
Abstract
As shown by the results of fluorescence spectrometric study on the interaction between 2,4,6-trichlorophenol and trypsin the quenching of the intrinsic fluorescence of trypsin by the presence of 2,4,6-trichlorophenol was a static quenching process due to the formation of a compound complex between the reactants through the actions of electrostatic and hydrophobic forces.The apparent binding constant K,number of binding sites and thermodynamic parameters of the reaction were calculated.Effects of 2,4,6-trichlorophenol on the configuration of trypsin and the enhancement of hydrophobicity of the residual radicals of tyrosin and tryptophan in the microenvironment were also studied by applying the techniques of synchronous fluorescence spectrometry and three dimensional fluorescence spectrometry.
中图分类号 O657
所属栏目 试验与研究
基金项目 江苏省高校自然科学基础研究项目(09KJD150007);江苏省“青蓝工程”资助项目
收稿日期 2009/12/8
修改稿日期
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备注周秋华(1953-),女,江苏盐城人,副教授,主要从事分析化学教学与科研工作.
引用该论文: ZHOU Qiu-hua,ZHANG Hong-mei,WEN Mei,WANG Yan-qing. Study on the Interaction of 2,4,6-Trichlorophenol and Trypsin by Fluorescence Spectrometry[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(10): 1109～1113
周秋华,张红梅,文美,王彦卿. 荧光光谱法研究2,4,6-三氯苯酚与胰蛋白酶的相互作用[J]. 理化检验－化学分册, 2010, 46(10): 1109～1113
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