提出了毛细管电泳法同时分离测定苷肽胶囊中3种核苷酸腺嘌呤-5′-单磷酸、胞嘧啶-5′-单磷酸和鸟嘌呤-5′-单磷酸含量的方法.以15 mmol·L-1硼砂+35 mmol·L-1碳酸钠+60 mmol·L-1羟丙基-β-环糊精混合溶液为电解质溶液,运行电压30 kV,于254 nm波长处进行紫外检测.3种核苷酸的线性范围依次为5.0~80.0,8.0~80.0,8.0~80.0 mg·L-1,检出限(3S/N)依次为2.0,3.0,3.0 mg·L-1.采用该方法对苷肽胶囊样品中3种核苷酸的含量分别进行了测定,所得加标回收率在92.1%~102.7%之间,相对标准偏差(n=5)均小于等于5.0%.

A method for the simultaneous separation and determination of 3 nucleotides,i.e.adenosine 5′-monophosphate (AMP),cytidine 5′-monophosphate (CMP) and guanosine 5′-monophosphate (GMP),in the health-care capsule containing glucoside and peptide (abbreviated as G-P capsule) by capillary electrophoresis was proposed.A mixture of solutions of 15 mmol·L-1 sodium borate,35 mmol·L-1 sodium carbonate and 60 mmol·L-1 hydroxypropyl-β-cyclodextrin was used as background electrolyte,and moving voltage of 30 kV was applied.UV-detection at the wavelength of 254 nm was adopted.Linearity ranges between 5.0-80.0 mg·L-1 (for AMP) and 8.0-80.0 mg·L-1 (for CMP and GMP) were obtained with detection limits (3S/N) of 2.0,3.0 and 3.0 mg·L-1 respectively.Recovery and precision of the method were tested by standard addition method,the results of recovery found were in the range of 92.1%-102.7% and RSD′s (n=5) were less than or equal to 5.0%.