反相高效液相色谱法测定血清中阿立哌唑及其代谢物
RP-HPLC Determination of Aripiprazole and its Metabolite in Blood Serum
摘 要
采用反相高效液相色谱法对血清中阿立哌唑及其代谢物脱氢阿立哌唑进行测定.血清样品经乙醚提取,所得提取液再经35 ℃氮气吹干,残留物经流动相溶解后,用Zorbax-C18色谱柱分离,用紫外检测器于254 nm波长处测定.阿立哌唑及其代谢物脱氢阿立哌唑的线性范围分别为4.0~2 000 μg·L-1和4.0~1 500 μg·L-1;测定下限(10S/N)均为4.0 μg·L-1.相对回收率分别为100.3%~102.7%和93.7%~103.9%;日内和日间相对标准偏差(n=5)均小于7%.
阿立哌唑
脱氢阿立哌唑
血清
RP-HPLC
Aripiprazole
Dehydro-aripiprazole
Blood serum
Abstract
A method of RP-HPLC for determination of aripiprazole and its metabolite,dehydro-aripiprazole was proposed.Sample of blood serum was extracted with ethyl ether and the eluate obtained was evaporated to dryness by N2-blowing at 35 ℃.The residue was dissolved with the mobile phase mixture and separated by passing through Zorbax-C18 column,and the eluate from the column was used for HPLC analysis with UV detector at the wavelength of 254 nm.Linearity ranges of aripiprazole and dehydro-aripiprazole were kept from 4.0 to 2 000 μg·L-1 and 4.0 to 1 500 μg·L-1 respectively,with same lower limits of determination (10S/N) of 4.0 μg·L-1.Values of relative recovery of aripiprazole and dehydro-aripiprazole in the range of 100.3%-102.7% and 93.7%-103.9% were found respectively.Values of intraday and interday relative standard deviation (n=5) were all less than 7%.
中图分类号 O652.63
所属栏目 工作简报
基金项目 国家重点基础研究发展计划(2007CB512306)
收稿日期 2010/3/5
修改稿日期
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备注任娟娟(1981-),女,河南人,研究实习员,硕士,主要从事精神药物分析及生化研究.
引用该论文: REN Juan-juan,LIN Zhi-guang,CUI Hui-ru,WENG Yi-ren,JIANG Ji-cheng,ZHUANG Dong-mei,LI Chun-bo. RP-HPLC Determination of Aripiprazole and its Metabolite in Blood Serum[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2010, 46(11): 1293～1295
任娟娟,林治光,崔慧茹,翁毅仁,蒋继承,庄冬梅,李春波. 反相高效液相色谱法测定血清中阿立哌唑及其代谢物[J]. 理化检验－化学分册, 2010, 46(11): 1293～1295
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【2】张晓红,白延.四川精神卫生[J].简述新型抗精神病药阿立哌唑,2006,19(4):163-164．
【3】WOOD M D,SCOTT C.Aripiprazole and its human metabolite are partial agonists at the human dopamine D2 receptor,but the rodent metabolite displays antagonist properties[J].Eur J Pharmacol,2006,546(1/3):88-94．
【4】MUSENGAA A,SARACINOA M A.Analysis of the recent antipsychotic aripiprazole in human plasma by capillary electrophoresis and high-performance liquid chromatography with diode array detection[J].Anal Chim Acta,2008,612(2):204-211．
【5】KUBO M,MIZOOKU Y.Development and validation of an LC-MS/MS method for the quantitative determination of aripiprazole and its main metabolite,OPC-14857,in human plasma[J].Chromatogr B,2005,822(1/2):294-299．
【6】SONG M,XU X X.Development of an LC-MS/MS method for the simultaneous quantification of aripiprazole and dehydroaripiprazole in human plasma[J].Anal Biochem,2009,385(2):270-277．
【7】LANCELIN F,DJEBRANI K.Development and validation of a high-performance liquid chromatography method using diode array detection for the simultaneous quantification of aripiprazole and dehydro-aripiprazole in human plasma[J].Chromatogr B,2008,867(1):15-19．
【8】SHIMOKAWA Y,AKIYAMA H.High performance liquid chromatographic methods for the determination of aripiprazole with ultraviolet detection in rat plasma and brain: Application to the pharmacokinetic study[J].Chromatogr B,2005,821(1):8-14．
【9】LI K Y,ZHOU Y G.Ultra-performance liquid chromatography-tandem mass spectrometry for the determination of atypical antipsychotics and some metabolites in vitro samples[J].Chromatogr B,2007,850(1/2):581-585．
【10】KIRCHHERR H,KHN-VELTEN W N.Quantitative determination of forty-eight antidepressants and antipsychotics in human serum by HPLC tandem mass spectrometry: A multi-level,single-sample approach[J].Chromatogr B,2006,843(1):100-113．
【11】KUBO M,MIZOOKU Y.Development and validation of an LC-MS/MS method for the quantitative determination of aripiprazole and its main metabolite,OPC-14857,in human plasma[J].Chromatogr B,2005,822(1/2):294-299．
【12】CHOONG E,RUDAZ S.Therapeutic drug monitoring of seven psychotropic drugs and four metabolites in human plasma by HPLC-MS[J].Pharm Biomed Anal,2009,50(5):1000-1008．
【13】ZUO X C,WANG F.LC-ESI-MS for rapid and sensitive determination of aripiprazole in human plasma[J].Chromatographia,2006,64(7/8):387-391．
【14】KIRSCHBAUM K M,MUELLER M J.Therapeutic monitoring of aripiprazole by HPLC with column-switching and spectrophotometric detection[J].Clin Chem,2005,51(9):1718-1721．
【15】HUANG H C,LIU C H.Detection and quantification of aripiprazole and its metabolite,dehydroaripiprazole,by gas chromatography-mass spectrometry in blood samples of psychiatric patients[J].Chromatogr B,2007,856(1/2):57-61．
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