建立反相高效液相色谱二极管阵列检测法同时测定丹参饮片中5种水溶性成分丹参素、原儿茶酸、原儿茶醛、迷迭香酸和丹酚酸B的含量.采用DiamonsilTM C18色谱柱(250 mm×4.6 mm,5 μm),以甲醇和乙酸(5+95)溶液为流动相进行梯度洗脱,流量1.0 mL·min-1,柱温30 ℃,检测波长298 nm.在此色谱条件下5种水溶性成分可完全分离.丹参素、原儿茶酸、原儿茶醛、迷迭香酸和丹酚酸B的线性范围分别为27.4~191.8,0.742~5.194,0.526 5~3.683,2.268~15.88,7.728~54.10 mg·L-1,平均回收率分别为101.5%,100.0%,100.3%,101.0%,102.3%.按此法分析了不同地区出产的丹参药材,结果显示:所测药材中5种成分的含量有显著差异.

RPHPLC was applied to the determination of 5 water soluble components,namely,danshensu,protocatechuic acid,protocatechuic aldehyde,rosmarinic acid and salvianolic acid B,in the TCM of sliced roots of Salvia miltiorrhiza.The sample was extracted with water and the extract was used for the determination.DiamonsilTM C18 column (250 mm×4.6 mm,5 μm) was used as stationary phase with column temperature at 30 ℃,and mixtures of methanol and dil.acetic acid (5+95) in different ratios were used as mobile phase in gradient elution with flow-rate of 1.0 mL·min-1.Wavelength of 298 nm was chosen for UV-detection of the 5 components with the DAD.Well separation was attained and linearity ranges were obtained between 27.4-191.8 mg·L-1 (for danshensu),0.742-5.194 mg·L-1 (for protocatechuic acid),0.526 5-3.683 mg·L-1 (for protocatechuic aldehyde),2.268-15.88 mg·L-1 (for rosmarinic acid),7.728-54.10 mg·L-1 (for salvianolic acid B).Values of average recovery found were 101.5%,100.0%,100.3%,101.0%,102.3% respectively.Significant differences among the contents of the 5 components were found in samples grown in different areas in our country as shown by the analytical results obtained by this method.