离子色谱法测定葡萄酒中的山梨酸和苯甲酸的含量
Determination of Sorbic Acid and Benzoic Acid in Wine by Ion Chromatography
摘 要
采用离子色谱法快速测定葡萄酒中的山梨酸和苯甲酸的含量。样品经超纯水稀释20倍,用聚酰胺粉末吸附提取溶液中的色素,净化液在IonPac AS11-HC阴离子色谱柱上分离,以30 mmol·L-1氢氧化钾溶液为淋洗液,采用抑制电导检测器检测,外标法定量。山梨酸和苯甲酸的质量浓度均在0.5~50 mg·L-1范围内与峰面积呈线性关系,方法的检出限(3S/N)在1.4~1.7 mg·L-1之间。加标回收率在97.7%~104%之间,测定值的相对标准偏差(n=6)在2.4%~3.4%之间。
山梨酸
苯甲酸
葡萄酒
Ion chromatography
Sorbic acid
Benzoic acid
Wine
Abstract
Ion chromatography was applied to the rapid determination of sorbic acid and benzoic acid in wine. The sample was diluted 20 times with ultrapure water, and the pigment in the solution was adsorbed and extracted with polyamide powder. The purified solution was separated on an IonPac AS11-HC anion chromatographic column with 30 mmol·L-1 KOH solution as eluent. The analytes were detected by suppressed conductivity detector. External standard method was used for quantification. Linear relationship were found between the values of peak areas and the mass concentration of both sorbic acid and benzoic acid in the same range of 0.5-50 mg·L-1, with detection limits (3S/N) in the range of 1.4-1.7 mg·L-1. Values of recovery rates obtained by standard addition method were in the range of 97.7%-104% and RSDs (n=6) were in the range of 2.4%-3.4%.
中图分类号 O657.7 DOI 10.11973/lhjy-hx201512006
所属栏目 工作简报
基金项目 福建省科技厅青年创新项目(2012J05020)
收稿日期
修改稿日期 2014/12/10
网络出版日期
作者单位点击查看
联系人作者陈志涛(chemczt@163.com)
备注陈志涛(1981-),男,福建南靖人,工程师,硕士研究 生,主要从事食品中农兽药残留和食品添加剂分析。
引用该论文: CHEN Zhi-tao,ZHANG Rui,ZHENG Xiang-ping,LIU Mei-hua,CHEN Chun-tian,HUANG Jing-jing. Determination of Sorbic Acid and Benzoic Acid in Wine by Ion Chromatography[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(12): 1658～1660
陈志涛,张睿,郑香平,刘美华,陈春添,黄菁菁. 离子色谱法测定葡萄酒中的山梨酸和苯甲酸的含量[J]. 理化检验－化学分册, 2015, 51(12): 1658～1660
共有人对该论文发表了看法，其中：
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】ZENGIN N, YVZBASIOGLU D, VNAL F, et al. The evaluation of the genotoxicity of two food preservatives: sodium benzoate and potassium benzoate［J］. Food Chem Toxicol, 2011,49:763-769.
【2】PARK H W, PARK E H, YUN H M, et al. Sodium benzoate-mediated cytotoxicity in mammalian cells［J］. J Food Biochem, 2011,35:1034-1046.
【3】MAMUR S, YVZBASIOGLU D, VNAL F, et al. Genotoxicity of food preservative sodium sorbate in human lymphocytes in vitro［J］. Cytotechnology, 2012,64:553-562.
【4】GB 15307-2006 葡萄酒［S］.
【5】XUE Yong, CHEN Na, LUO Chun-ying, et al. Simultaneous determination of seven preservatives in cosmetics by dispersive liquid-liquid microextraction coupled with high performance capillary electrophoresis［J］. Anal Methods, 2013,5:2391-2397.
【6】HSU S H, HU C C, CHIU T C. Online dynamic pH junction-sweeping for the determination of benzoic and sorbic acids in food products by capillary electrophoresis［J］. Anal Bioanal Chem, 2014,406:635-641.
【7】SUN Yang, WANG Xiao-qing, HUANG Yi-lei, et al. Derivatization following hollow-fiber microextraction with tetramethylammonium acetate as a dual-function reagent for the determination of benzoic acid and sorbic acid by GC［J］. J Sep Sci, 2013,36:2268-2276.
【8】朱国强,韦丽丽,江恩源.多样品同时萃取-气相色谱法测定食品中山梨酸和苯甲酸［J］.现代食品科技, 2013,29(5):1166-1169.
【9】KAMANKESH M, MOHAMMADI A, TEHRANI Z M, et al. Dispersive liquid-liquid microextraction followed by high-performance liquid chromatography for determination of benzoate and sorbate in yogurt drinks and method optimization by central composite design［J］. Talanta, 2013,109:46-51.
【10】周红霞,张红琳,黄娟,等.高效液相色谱法同时检测液体食品中苯甲酸、山梨酸、安赛蜜、糖精钠含量的研究［J］.中国食品添加剂, 2014(3):208-212.
【11】田玉平,舒晓莲,顾玲玲,等.高效液相色谱法测定含乳饮料中苯甲酸、山梨酸钾、水杨酸［J］.化学世界, 2013,53(1):24-25.
【12】马国军.离子色谱法测定饮料中7种食品添加剂［J］.理化检测-化学分册, 2014,50(6):766-768.
【2】PARK H W, PARK E H, YUN H M, et al. Sodium benzoate-mediated cytotoxicity in mammalian cells［J］. J Food Biochem, 2011,35:1034-1046.
【3】MAMUR S, YVZBASIOGLU D, VNAL F, et al. Genotoxicity of food preservative sodium sorbate in human lymphocytes in vitro［J］. Cytotechnology, 2012,64:553-562.
【4】GB 15307-2006 葡萄酒［S］.
【5】XUE Yong, CHEN Na, LUO Chun-ying, et al. Simultaneous determination of seven preservatives in cosmetics by dispersive liquid-liquid microextraction coupled with high performance capillary electrophoresis［J］. Anal Methods, 2013,5:2391-2397.
【6】HSU S H, HU C C, CHIU T C. Online dynamic pH junction-sweeping for the determination of benzoic and sorbic acids in food products by capillary electrophoresis［J］. Anal Bioanal Chem, 2014,406:635-641.
【7】SUN Yang, WANG Xiao-qing, HUANG Yi-lei, et al. Derivatization following hollow-fiber microextraction with tetramethylammonium acetate as a dual-function reagent for the determination of benzoic acid and sorbic acid by GC［J］. J Sep Sci, 2013,36:2268-2276.
【8】朱国强,韦丽丽,江恩源.多样品同时萃取-气相色谱法测定食品中山梨酸和苯甲酸［J］.现代食品科技, 2013,29(5):1166-1169.
【9】KAMANKESH M, MOHAMMADI A, TEHRANI Z M, et al. Dispersive liquid-liquid microextraction followed by high-performance liquid chromatography for determination of benzoate and sorbate in yogurt drinks and method optimization by central composite design［J］. Talanta, 2013,109:46-51.
【10】周红霞,张红琳,黄娟,等.高效液相色谱法同时检测液体食品中苯甲酸、山梨酸、安赛蜜、糖精钠含量的研究［J］.中国食品添加剂, 2014(3):208-212.
【11】田玉平,舒晓莲,顾玲玲,等.高效液相色谱法测定含乳饮料中苯甲酸、山梨酸钾、水杨酸［J］.化学世界, 2013,53(1):24-25.
【12】马国军.离子色谱法测定饮料中7种食品添加剂［J］.理化检测-化学分册, 2014,50(6):766-768.
相关信息
