超高效合相色谱法测定化妆品中7种防晒剂
UPC2 Determination of 7 Sunscreeners in Cosmetics
摘 要
采用超高效合相色谱法(UPC2)分离和测定化妆品中的7种防晒剂。样品经甲醇-水(8+2)混合液超声提取后,选用Waters Hss C18 SB色谱柱分离,以超临界二氧化碳-甲醇混合物为流动相进行梯度洗脱。紫外检测波长为276 nm和310 nm。7种防晒剂的线性范围均为1.0~80.0 mg·L-1,检出限(3S/N)均为200 mg·kg-1。加标回收率在90.2%~103%之间,测定值的相对标准偏差(n=6)在1.6%~5.2%之间。
防晒剂
化妆品
Ultra high performance convergence chromatography
Sunscreeners
Cosmetics
Abstract
Ultra high performance convergence chromatography UPC2 was applied to the determination of 7 sunscreeners in cosmetics. The sample was extracted ultrasonically with a mixture of CH3OH-H2O (8+2). The extract was separated on Waters Hss C18 SB chromatographic column, with a mixture of supercritical CO2 and methanol as mobile phase in the gradient elution. UV detection was made at the wavelengths of 276 nm and 310 nm. Linearity ranges of the 7 sunscreeners were same in the range of 1.0-80.0 mg·L-1, with same detection limits (3S/N) of 200 mg·kg-1. Values of recovery obtained by standard addition method were in the range of 90.2%-103%, with RSDs (n=6) in the range of 1.6%-5.2%.
中图分类号 O657.7 DOI 10.11973/lhjy-hx201512008
所属栏目 工作简报
基金项目 海南省重点科技计划项目(ZDXM2014083)
收稿日期 2014/12/11
修改稿日期
网络出版日期
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备注徐莉(1981-),女,海南海口人,工程师,研究方向 为食品有害物质检测。
引用该论文: XU Li,PANG Dao-biao,FU Ling-mei,HUANG Xin-ying. UPC2 Determination of 7 Sunscreeners in Cosmetics[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2015, 51(12): 1666～1669
徐莉,庞道标,符灵梅,黄薪颖. 超高效合相色谱法测定化妆品中7种防晒剂[J]. 理化检验－化学分册, 2015, 51(12): 1666～1669
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参考文献
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【4】来国桥,张建兴,卢良.高效液相色谱法分离测定化妆品中三种紫外吸收剂的含量［J］.香料香精化妆品, 2003,6(3):22-26.
【5】张泓,周世伟,吴卫平.高效液相色谱法测定化妆品中的防晒剂［J］.中国卫生检验杂志, 2000,6(3):151-153.
【6】于成峰.高效液相色谱法同时测定羟苯甲酮-5-磺酸、水杨酸钠、羟苯甲酮、水杨酸苯酯［J］.精细石油化工, 1999,7(4):64-65.
【7】姚孝元,郑星泉,秦效英,等.反相高效液相色谱法测定化妆品中的防晒剂［J］.色谱, 1998,16(3):223-225.
【8】虞成华,巢强国,曹程明,等.高效液相色谱法测定化妆品中防晒剂［J］.理化检验-化学分册, 2010,46(11):1273-1275.
【9】许秀敏,高燕红,龙朝阳,等.高效液相色谱法测定化妆品中15种化学防晒剂［J］.中国卫生检验杂志, 2011,21(7):1601-1605.
【10】马杰,林维宣.反相高效液相色谱法测定化妆品中的几种防晒剂［J］.日用化学工业, 2010,40(3):225-228.
【11】GOURMEL C, ALEXANDRE G P, WALLER L, et al. Evaluation and comparison of various separation techniques for the analysis of closely-related compounds of pharmaceutical interest［J］. Journal of Chromatography A, 2013,1282:172-177.
【12】PERRENOUD A G, VEUTJEY J L, GUILLARME D. Analysis of basic compounds by supercritical fluid chromatography: attempts to improve peak shape and maintain mass spectrometry compatibility［J］. Journal of Chromatography A, 2012,1266:158-167.
【13】徐永威,孙庆龙,黄静,等.沃特世超高效合相色谱系统-ACQUITY UPC2［J］.现代科学仪器, 2012,10(5):153-153.
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