采用高效液相色谱法测定了猪肉及血清中盐酸克伦特罗残留量。样品经甲醇-盐酸混合液提取,乙二胺-N-丙基硅烷净化后,采用Zorbax Eclipse C18色谱柱分离,以甲醇-0.01 mol·L-1磷酸二氢钠(68+32)混合液为流动相进行洗脱,在波长270 nm处进行紫外检测。盐酸克伦特罗的线性范围为0.01~5.00 mg·L-1,检出限(3S/N)为1.8 μg·kg-1,加标回收率在87.4%~99.2%之间,测定值的相对标准偏差(n=5)在2.1%~2.8%之间。

HPLC was applied to the determination of residual amounts of clenbuterol in pork and serum. The sample was extracted with a mixture of methanol and 0.01 mol·L-1 hydrochloric acid (68+32) and after purification with ethylenediamine-N-propylsilane, the sample solution was separated on Zorbax Eclipse C18 chromatographic column with a mixture of methanol and sodium dihydrogen phosphate solution (68+32) as mobile phase. UV detection was made at the wavelength of 270 nm. Linearity range of clenbuterol was between 0.01-5.00 mg·L-1, with detection limit (3S/N) of 1.8 μg·kg-1. Values of recovery obtained by standard addition method were in the range of 87.4%-99.2% , with RSDs (n=5) in the range of 2.1%-2.8%.