HPLC Determination of 3 Furfurals in Hydrothermal Carbonization Solution of Camellia Oleifera Shells
摘 要
采用高效液相色谱法同时测定油茶籽壳水热碳化液中的5-羟甲基糠醛、糠醛和5-甲基糠醛的含量。油茶籽壳水热碳化液经Thermo ODS色谱柱分离,以甲醇-水混合液作为流动相进行梯度洗脱,采用二极管阵列检测器,检测波长为285 nm。3种糠醛类物质的质量浓度在一定范围内与其峰面积呈线性关系,5-羟甲基糠醛、糠醛和5-甲基糠醛的检出限(3S/N)分别为0.02,0.005,0.02 mg·L-1,测定下限(10S/N)分别为0.04,0.02,0.05 mg·L-1。加标回收率分别在74.9%~101%,94.5%~109%和98.6%~112%之间,测定值的相对标准偏差小于10%。
Abstract
HPLC was applied for the determination of 5-hydroxymethyl-2-furfural, 2-furaldehyde and 5-methyl-2-furaldehyde in hydrothermal carbonization solution of camellia oleifera shells. The solution was separated on a Thermo ODS chromatographic column, with a mixture of methanol and water as mobile phase for gradient elution. DAD detector was used with the detection wavelength of 285 nm. Linear relationships were found between the peak areas and the mass concentrations of the 3 furfurals in definite ranges. The detection limits (3S/N) of 5-hydroxymethyl-2-furfural, 2-furaldehyde and 5-methyl-2-furaldehyde were 0.02, 0.005, 0.02 mg·L-1, respectively, and the lower limits of determination (10S/N) were 0.04, 0.02, 0.05 mg·L-1, respectively. Recovery rates obtained by standard addition method were in the ranges of 74.9%-101%, 94.5%-109% and 98.6%-112%, respectively, and RSDs were less than 10%.
中图分类号 O657.7 DOI 10.11973/lhjy-hx201607010
所属栏目 工作简报
基金项目 国家自然科学基金项目(21165013;21366024);江西 省青年科学基金项目(20114BAB213016);江西省重大科技招标项 目(赣科发计字[2014]56-6号);南昌航空大学研究生创新专项资 金项目(YC2015014)
收稿日期 2015/6/29
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备注马文天(1992-),男,河南洛阳人,硕士研究生,研究 方向为生物质废弃物资源化与利用。
引用该论文: MA Wen-tian,GUO Hui-qin,YAN Liu-shui,LI Ke-xin,LIU Yu,LIU Shu-fen. HPLC Determination of 3 Furfurals in Hydrothermal Carbonization Solution of Camellia Oleifera Shells[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2016, 52(7): 787~791
马文天,郭会琴,颜流水,李可心,刘 宇,刘淑芬. 高效液相色谱法测定油茶籽壳水热碳化液中的3种糠醛类物质[J]. 理化检验-化学分册, 2016, 52(7): 787~791
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【4】冯培良,王君,陈明强,等.生物质水解制备糠醛的研究进展[J].化学与生物工程, 2014,31(8):6-9.
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【6】郑连姬,冯璨,杜渝,等.蜂蜜中羟甲基糠醛的近红外光谱快速测定方法[J].食品工业科技, 2012,33(16):89-91.
【7】王建刚,李丽.反相高效液相色谱法测定白酒中糠醛[J].理化检验-化学分册, 2010,46(12):1470-1471.
【8】汪辉,黎瑛,夏立新,等.高效液相色谱法测定葡萄干中糠醛类物质[J].理化检验-化学分册, 2014,50(11):1377-1381.
【9】高夫超,崔长日,魏月,等.高效液相色谱法测定蜂王浆中6种糠醛类物质含量[J].食品安全质量检测学报, 2014,5(11):3603-3609.
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【11】ARIFFIN A, GHAZALI H M, KAVOUSI P. Validation of a HPLC method for determination of hydroxymethylfurfural in crude palm oil[J]. Food Chemistry, 2014,154(1):102-107.
【12】RAMREZ-JIMNEZ A, GARA-VILLANOVA B, GUERRA-HERNNDEZ E. Hydroxymethylfurfural and methylfurfural content of selected bakery products[J]. Food Research International, 2000,33(10):833-838.
【13】JBSTL D, HUSY T, ALEXANDER J, et al. Analysis of 5-hydroxymethyl-2-furoic acid(HMFA) the main metabolite of alimentary 5-hydroxymethyl-2-furfural(HMF) with HPLC and GC in urine[J]. Food Chemistry, 2010,123(3):814-818.
【14】RUIZ-MATUTE A I, RODRGUEZ-SNCHEZ S, SANZ M L, et al. Detection of adulterations of honey with high fructose syrups from inulin by GC analysis[J]. Journal of Food Composition and Analysis, 2010,23(3):273-276.
【15】江伟,李红,周梅,等.采用GC-MS同时检测啤酒中的醛酯类老化物质[J].食品与发酵工业, 2010,36(10):153-158.
【16】杨朝霞,李梅,董建军,等.高效液相色谱同时测定啤酒中的5-羟甲基糠醛和糠醛[J].酿酒科技, 2006(9):88-90.
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