固样（2.000 0 g）加水10.0 mL使其超声分散（液样取2.00 mL，加水8.0 mL），加甲醇稀释至50.0 mL。取上清液2.00 mL，用0.1 mol·L^-1盐酸溶液稀释至10.0 mL。所得溶液经Cleanert PCX混合型阳离子固相萃取柱净化、氨水-甲醇（5+95）混合液洗脱。洗出液进行色谱分离，用ZORBAX SB-C₁₈色谱柱作固定相，并以不同体积比的乙腈和0.01 mol·L^-1乙酸铵溶液（pH 3.5）的混合液为流动相进行梯度淋洗。质谱分析中，采用电喷雾正离子源和多反应监测模式检测。13种壮阳类化合物的质量浓度在1.0~1 000 μg·L^-1范围内呈线性，方法的检出限（3S/N）在3.0~40 μg·kg^-1之间。加标回收率为80.1%~111%，相对标准偏差（n=6）小于8%。

Solid sample (2.000 0 g) was dispersed ultrasonically in 10.0 mL of water, or 2.00 mL of liquid sample was mixed with 8.0 mL of water, and then diluted to 50.0 mL with CH₃OH. 2.00 mL of the supernatant was taken and diluted to 10.0 mL with 0.1 mol·L^-1 HCl solution, and then purified by passing through Cleanert PCX mixed cationic SPE column, which was then eluted with a mixture of aq. ammonia and methanol (5+95). The eluate was separated on ZORBAX SB-C₁₈ column using mixtures of 0.01 mol·L^-1 NH₄OAc solution (pH 3.5) and acetonitrile in various ratios as mobile phase in gradient elution. ESI⁺ and MRM were adopted in MS. Linear relationships between values of peak areas and mass concentrations of the 13 forbidden drugs were obtained in the same range of 1.0-1 000 μg·L^-1, with values of detection limits (3S/N) in the range of 3.0-40 μg·kg^-1. Tests for recovery were made by standard addition method, giving values of recovery in the range of 80.1%-111%, with values of RSDs (n=6) less than 8%.