MWCNTS/BTB Modified Glassy Carbon Electrode Determination of Adenine in Cordyceps Sinensis
摘 要
用循环伏安法(CV)于磷酸盐缓冲溶液中在-0.8~1.6 V电位间扫描15周,使溴百里酚蓝(BTB)聚合于玻碳电极表面,制得BTB/GCE电极。另取经羰基化的多壁碳纳米管10 mg分散于10 mL DMF中,并将此分散液适量滴于BTB/GCE电极表面,于红外灯中烘干后即得MWCNTS/BTB/GCE修饰电极,用于测定冬虫夏草中腺嘌呤含量的传感器。通过CV研究表明:该传感器在pH 6.8的磷酸盐缓冲溶液中,扫描速率为100 mV·s-1的条件下对腺嘌呤的响应明显,且腺嘌呤的质量浓度在0.05~1.0 g·L-1范围内与相应的氧化峰电流呈线性关系,其检出限(3s)为0.025 g·L-1。该方法用于5个不同批次的样品的分析,测得腺嘌呤含量与其标示量相符,测定值的相对标准偏差(n=5)在0.8%~2.0%之间。
Abstract
Bromothymol blue (BTB) was polymerized on the surface of GCE in a phosphate buffer solution by CV in the potential range of -0.8 to 1.6 V, with scanning for 15 cycles, to form BTB modified GCE. A portion of 10 mg of carbonylated MWCNTS was dispersed in 10 mL of DMF, and an appropriate amount of the dispersion was dropped on the surface of the BTB/GCE, forming the modified electrode of MWCNTS/BTB/GCE, which was dried under ultra-red lamp. As shown by the CV study, significant response of adenine at the MWCNTS/BTB/GCE was observed in phosphate buffer at pH 6.8 with scanning rate of 100 mV·s-1. It was proved that the modified GCE was suitable to be used as a sensor for CV determination of adenine in Cordyceps sinensis. Linear relationship between values of oxidative peak current and mass concentration of adenine was obtained in the range of 0.05-1.0 g·L-1, with detection limit (3s) of 0.025 g·L-1. 5 samples taken from different batches were taken and analyzed by the proposed method, giving results in consistency with the labelled values. Values of RSDs (n=5) found were in the range of 0.8%-2.0%.
中图分类号 O657.1 DOI 10.11973/lhjy-hx201701012
所属栏目 工作简报
基金项目
收稿日期 2015/12/9
修改稿日期
网络出版日期
作者单位点击查看
联系人作者申贵隽(sgj0501@163.com)
备注冯宇婷(1992-),女,河南商丘人,硕士研究生,主要研究方向为电分析化学。
引用该论文: FENG Yu-ting,SHEN Gui-jun,HUANG Hui-juan. MWCNTS/BTB Modified Glassy Carbon Electrode Determination of Adenine in Cordyceps Sinensis[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2017, 53(1): 60~63
冯宇婷,申贵隽,黄慧娟. 溴百里酚蓝/多壁碳纳米管修饰玻碳电极测定冬虫夏草中腺嘌呤[J]. 理化检验-化学分册, 2017, 53(1): 60~63
共有人对该论文发表了看法,其中:
人认为该论文很差
人认为该论文较差
人认为该论文一般
人认为该论文较好
人认为该论文很好
参考文献
【1】申远,陈乐玥,华欣,等.碳纳米管修饰电极测定冬虫夏草有效成分含量[J].中国无机分析化学, 2012,2(4):78-81.
【2】国家药典委员会.中国药典:一部[M].北京:化学工业出版社, 2005.
【3】TANG C, YOGESWARAN U, CHEN S M.Simultaneous determination of adenine,guanine and thymine at multiwalled carbon nanotubes incorporated with poly (new fuchsin) composite film[J]. Analytical Chimica Acta, 2009,636(1):19-27.
【4】夏雅淋,邓春艳,向娟.基于BCNTs/GC电极的鸟嘌呤与腺嘌呤电化学行为及其同时检测[J].电化学, 2012(4):365-370.
【5】TODD B, ZHAO J, FLEET G. HPLC measurement of guanine for the determination of nucleic acids (RNA) in yeasts[J]. Journal of Microbiological Methods, 1995,22(1):1-10.
【6】XU D K, HUA L, CHEN H Y. Determination of purine bases by capillary zone electrophoresis with wall-jet amperometric detection[J]. Analytical Chimica Acta, 1996,335(1/2):95-101.
【7】丁亮,罗嵩,赵月朝.ICP-MS法测定管材中金属释放量的实验室研究[J].中国卫生检验杂志, 2005,15(2):166-167.
【8】牛凌梅,张玉娜,连靠奇,等.DNA固定纳米金修饰的玻碳电极对腺嘌呤的测定及应用研究[J].中国卫生检验杂志, 2014(1):30-31.
【9】王光灿,师真,朱光辉,等.碳纳米管修饰电极在分析化学中的应用[J].理化检验-化学分册, 2008,44(8):801-806.
【10】侯晓兰,申贵隽,刘倩,等.β-环糊精/碳纳米管修饰电极上盐酸异丙嗪的电化学行为研究[J].分析科学学报, 2011,27(5):603-606.
【11】康伟忠,申贵隽,王荣海,等.盐酸二甲双胍在β-CD/MWNTs修饰玻碳电极上的电化学行为研究[J].分析试验室, 2015,34(1):99-103.
【2】国家药典委员会.中国药典:一部[M].北京:化学工业出版社, 2005.
【3】TANG C, YOGESWARAN U, CHEN S M.Simultaneous determination of adenine,guanine and thymine at multiwalled carbon nanotubes incorporated with poly (new fuchsin) composite film[J]. Analytical Chimica Acta, 2009,636(1):19-27.
【4】夏雅淋,邓春艳,向娟.基于BCNTs/GC电极的鸟嘌呤与腺嘌呤电化学行为及其同时检测[J].电化学, 2012(4):365-370.
【5】TODD B, ZHAO J, FLEET G. HPLC measurement of guanine for the determination of nucleic acids (RNA) in yeasts[J]. Journal of Microbiological Methods, 1995,22(1):1-10.
【6】XU D K, HUA L, CHEN H Y. Determination of purine bases by capillary zone electrophoresis with wall-jet amperometric detection[J]. Analytical Chimica Acta, 1996,335(1/2):95-101.
【7】丁亮,罗嵩,赵月朝.ICP-MS法测定管材中金属释放量的实验室研究[J].中国卫生检验杂志, 2005,15(2):166-167.
【8】牛凌梅,张玉娜,连靠奇,等.DNA固定纳米金修饰的玻碳电极对腺嘌呤的测定及应用研究[J].中国卫生检验杂志, 2014(1):30-31.
【9】王光灿,师真,朱光辉,等.碳纳米管修饰电极在分析化学中的应用[J].理化检验-化学分册, 2008,44(8):801-806.
【10】侯晓兰,申贵隽,刘倩,等.β-环糊精/碳纳米管修饰电极上盐酸异丙嗪的电化学行为研究[J].分析科学学报, 2011,27(5):603-606.
【11】康伟忠,申贵隽,王荣海,等.盐酸二甲双胍在β-CD/MWNTs修饰玻碳电极上的电化学行为研究[J].分析试验室, 2015,34(1):99-103.
相关信息